Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20580 and BPHYT_RS20585 are separated by 229 nucleotides BPHYT_RS20585 and BPHYT_RS20590 are separated by 132 nucleotides BPHYT_RS20590 and BPHYT_RS20595 are separated by 162 nucleotides BPHYT_RS20595 and BPHYT_RS20600 are separated by 85 nucleotides
BPHYT_RS20580: BPHYT_RS20580 - NADH dehydrogenase, at 177,919 to 179,145
_RS20580
BPHYT_RS20585: BPHYT_RS20585 - MarR family transcriptional regulator, at 179,375 to 179,836
_RS20585
BPHYT_RS20590: BPHYT_RS20590 - methionine sulfoxide reductase A, at 179,969 to 180,475
_RS20590
BPHYT_RS20595: BPHYT_RS20595 - hypothetical protein, at 180,638 to 180,910
_RS20595
BPHYT_RS20600: BPHYT_RS20600 - peroxiredoxin, at 180,996 to 181,421
_RS20600
Position (kb) 179
180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 178.991 kb on + strand, within BPHYT_RS20580 at 178.992 kb on - strand, within BPHYT_RS20580 at 178.992 kb on - strand, within BPHYT_RS20580 at 179.033 kb on - strand at 179.033 kb on - strand at 179.237 kb on - strand at 180.372 kb on + strand, within BPHYT_RS20590 at 180.373 kb on - strand, within BPHYT_RS20590 at 180.373 kb on - strand, within BPHYT_RS20590 at 180.390 kb on - strand, within BPHYT_RS20590 at 180.721 kb on - strand, within BPHYT_RS20595 at 180.816 kb on + strand, within BPHYT_RS20595 at 181.012 kb on - strand at 181.240 kb on + strand, within BPHYT_RS20600 at 181.240 kb on + strand, within BPHYT_RS20600 at 181.240 kb on + strand, within BPHYT_RS20600 at 181.241 kb on - strand, within BPHYT_RS20600
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 178,991 + BPHYT_RS20580 0.87 +1.1 178,992 - BPHYT_RS20580 0.87 +1.4 178,992 - BPHYT_RS20580 0.87 -0.3 179,033 - +0.7 179,033 - -2.3 179,237 - +1.0 180,372 + BPHYT_RS20590 0.79 -2.7 180,373 - BPHYT_RS20590 0.80 -0.6 180,373 - BPHYT_RS20590 0.80 -2.6 180,390 - BPHYT_RS20590 0.83 -0.8 180,721 - BPHYT_RS20595 0.30 +0.0 180,816 + BPHYT_RS20595 0.65 -0.4 181,012 - -1.5 181,240 + BPHYT_RS20600 0.57 -0.6 181,240 + BPHYT_RS20600 0.57 +0.2 181,240 + BPHYT_RS20600 0.57 -2.8 181,241 - BPHYT_RS20600 0.58 -1.6
Or see this region's nucleotide sequence