Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20215 and BPHYT_RS20220 are separated by 128 nucleotides BPHYT_RS20220 and BPHYT_RS20225 are separated by 87 nucleotides
BPHYT_RS20215: BPHYT_RS20215 - RNA polymerase sigma 70, at 80,806 to 81,477
_RS20215
BPHYT_RS20220: BPHYT_RS20220 - antibiotic synthesis protein MbtH, at 81,606 to 81,845
_RS20220
BPHYT_RS20225: BPHYT_RS20225 - taurine catabolism dioxygenase tauD/tfdA, at 81,933 to 82,967
_RS20225
Position (kb) 81
82 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 80.685 kb on - strand at 80.722 kb on + strand at 80.723 kb on - strand at 80.806 kb on - strand at 80.806 kb on - strand at 81.094 kb on + strand, within BPHYT_RS20215 at 81.094 kb on + strand, within BPHYT_RS20215 at 81.095 kb on - strand, within BPHYT_RS20215 at 81.507 kb on + strand at 81.664 kb on - strand, within BPHYT_RS20220 at 81.693 kb on + strand, within BPHYT_RS20220 at 81.933 kb on - strand at 82.029 kb on + strand at 82.097 kb on - strand, within BPHYT_RS20225 at 82.176 kb on + strand, within BPHYT_RS20225 at 82.221 kb on + strand, within BPHYT_RS20225 at 82.222 kb on - strand, within BPHYT_RS20225 at 82.222 kb on - strand, within BPHYT_RS20225 at 82.279 kb on - strand, within BPHYT_RS20225 at 82.302 kb on - strand, within BPHYT_RS20225 at 82.645 kb on + strand, within BPHYT_RS20225 at 82.729 kb on - strand, within BPHYT_RS20225 at 82.729 kb on - strand, within BPHYT_RS20225
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 80,685 - -0.2 80,722 + +1.8 80,723 - -0.2 80,806 - -1.7 80,806 - -2.6 81,094 + BPHYT_RS20215 0.43 +0.6 81,094 + BPHYT_RS20215 0.43 -0.4 81,095 - BPHYT_RS20215 0.43 +1.7 81,507 + -1.3 81,664 - BPHYT_RS20220 0.24 -3.2 81,693 + BPHYT_RS20220 0.36 -0.9 81,933 - +0.4 82,029 + +0.5 82,097 - BPHYT_RS20225 0.16 -3.5 82,176 + BPHYT_RS20225 0.23 +0.8 82,221 + BPHYT_RS20225 0.28 -2.1 82,222 - BPHYT_RS20225 0.28 -0.1 82,222 - BPHYT_RS20225 0.28 -1.8 82,279 - BPHYT_RS20225 0.33 -0.2 82,302 - BPHYT_RS20225 0.36 -0.3 82,645 + BPHYT_RS20225 0.69 +0.1 82,729 - BPHYT_RS20225 0.77 -0.6 82,729 - BPHYT_RS20225 0.77 -2.0
Or see this region's nucleotide sequence