Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15070 and BPHYT_RS15075 are separated by 121 nucleotides BPHYT_RS15075 and BPHYT_RS15080 are separated by 298 nucleotides
BPHYT_RS15070: BPHYT_RS15070 - amidase, at 3,428,442 to 3,429,821
_RS15070
BPHYT_RS15075: BPHYT_RS15075 - dihydrokaempferol 4-reductase, at 3,429,943 to 3,430,452
_RS15075
BPHYT_RS15080: BPHYT_RS15080 - FAD-binding molybdopterin dehydrogenase, at 3,430,751 to 3,432,382
_RS15080
Position (kb)
3429
3430
3431 Strain fitness (log2 ratio)
-1
0
1 at 3429.387 kb on + strand, within BPHYT_RS15070 at 3429.388 kb on - strand, within BPHYT_RS15070 at 3429.573 kb on - strand, within BPHYT_RS15070 at 3429.638 kb on + strand, within BPHYT_RS15070 at 3429.851 kb on + strand at 3429.852 kb on - strand at 3429.858 kb on + strand at 3429.870 kb on - strand at 3429.894 kb on + strand at 3430.034 kb on - strand, within BPHYT_RS15075 at 3430.220 kb on - strand, within BPHYT_RS15075 at 3430.220 kb on - strand, within BPHYT_RS15075 at 3430.667 kb on - strand at 3430.670 kb on + strand at 3430.671 kb on - strand at 3430.671 kb on - strand at 3430.730 kb on + strand at 3430.730 kb on + strand at 3430.730 kb on + strand at 3430.731 kb on - strand at 3430.731 kb on - strand at 3430.731 kb on - strand at 3430.735 kb on + strand at 3430.736 kb on + strand at 3430.736 kb on + strand at 3430.737 kb on - strand at 3430.737 kb on - strand at 3430.760 kb on + strand at 3430.760 kb on + strand at 3431.070 kb on + strand, within BPHYT_RS15080
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 3,429,387 + BPHYT_RS15070 0.68 +1.4 3,429,388 - BPHYT_RS15070 0.69 -0.8 3,429,573 - BPHYT_RS15070 0.82 -1.6 3,429,638 + BPHYT_RS15070 0.87 -0.8 3,429,851 + -1.0 3,429,852 - +0.4 3,429,858 + -0.5 3,429,870 - -0.1 3,429,894 + +0.5 3,430,034 - BPHYT_RS15075 0.18 +0.4 3,430,220 - BPHYT_RS15075 0.54 -0.3 3,430,220 - BPHYT_RS15075 0.54 +0.0 3,430,667 - +1.5 3,430,670 + +1.2 3,430,671 - -0.7 3,430,671 - -1.7 3,430,730 + -0.3 3,430,730 + -0.1 3,430,730 + +0.6 3,430,731 - -0.5 3,430,731 - -0.6 3,430,731 - -1.4 3,430,735 + -0.3 3,430,736 + +0.8 3,430,736 + -0.2 3,430,737 - -0.8 3,430,737 - -1.7 3,430,760 + +0.9 3,430,760 + -0.4 3,431,070 + BPHYT_RS15080 0.20 +0.5
Or see this region's nucleotide sequence