Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12530 and BPHYT_RS12535 are separated by 281 nucleotides BPHYT_RS12535 and BPHYT_RS12540 are separated by 44 nucleotides BPHYT_RS12540 and BPHYT_RS12545 are separated by 12 nucleotides
BPHYT_RS12530: BPHYT_RS12530 - ATP phosphoribosyltransferase regulatory subunit, at 2,860,864 to 2,862,015
_RS12530
BPHYT_RS12535: BPHYT_RS12535 - membrane protein, at 2,862,297 to 2,862,488
_RS12535
BPHYT_RS12540: BPHYT_RS12540 - membrane protein, at 2,862,533 to 2,863,435
_RS12540
BPHYT_RS12545: BPHYT_RS12545 - membrane protein, at 2,863,448 to 2,864,848
_RS12545
Position (kb)
2862
2863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2861.688 kb on + strand, within BPHYT_RS12530 at 2861.709 kb on - strand, within BPHYT_RS12530 at 2861.874 kb on + strand, within BPHYT_RS12530 at 2861.875 kb on - strand, within BPHYT_RS12530 at 2861.875 kb on - strand, within BPHYT_RS12530 at 2861.875 kb on - strand, within BPHYT_RS12530 at 2861.875 kb on - strand at 2861.876 kb on + strand, within BPHYT_RS12530 at 2862.001 kb on + strand at 2862.001 kb on + strand at 2862.001 kb on + strand at 2862.028 kb on - strand at 2862.382 kb on - strand, within BPHYT_RS12535 at 2862.496 kb on - strand at 2862.587 kb on - strand at 2862.676 kb on - strand, within BPHYT_RS12540 at 2863.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,861,688 + BPHYT_RS12530 0.72 +1.1 2,861,709 - BPHYT_RS12530 0.73 -0.1 2,861,874 + BPHYT_RS12530 0.88 +0.2 2,861,875 - BPHYT_RS12530 0.88 +0.2 2,861,875 - BPHYT_RS12530 0.88 -0.6 2,861,875 - BPHYT_RS12530 0.88 +0.4 2,861,875 - +3.1 2,861,876 + BPHYT_RS12530 0.88 -1.7 2,862,001 + -2.8 2,862,001 + -0.4 2,862,001 + -1.0 2,862,028 - +0.2 2,862,382 - BPHYT_RS12535 0.44 -2.7 2,862,496 - -1.7 2,862,587 - -1.6 2,862,676 - BPHYT_RS12540 0.16 +0.6 2,863,472 - +0.6
Or see this region's nucleotide sequence