Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12235

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12225 and BPHYT_RS12230 are separated by 222 nucleotidesBPHYT_RS12230 and BPHYT_RS12235 are separated by 143 nucleotidesBPHYT_RS12235 and BPHYT_RS12240 are separated by 90 nucleotides BPHYT_RS12225: BPHYT_RS12225 - carnitine dehydratase, at 2,792,280 to 2,793,323 _RS12225 BPHYT_RS12230: BPHYT_RS12230 - hypothetical protein, at 2,793,546 to 2,793,824 _RS12230 BPHYT_RS12235: BPHYT_RS12235 - DNA repair protein RadA, at 2,793,968 to 2,795,344 _RS12235 BPHYT_RS12240: BPHYT_RS12240 - alanine racemase, at 2,795,435 to 2,796,505 _RS12240 Position (kb) 2793 2794 2795 2796Strain fitness (log2 ratio) -3 -2 -1 0 1at 2792.974 kb on - strand, within BPHYT_RS12225at 2793.321 kb on + strandat 2793.322 kb on - strandat 2793.604 kb on + strand, within BPHYT_RS12230at 2793.693 kb on + strand, within BPHYT_RS12230at 2793.873 kb on + strandat 2793.894 kb on - strandat 2793.898 kb on + strandat 2793.898 kb on + strandat 2793.915 kb on + strandat 2793.915 kb on + strandat 2793.970 kb on - strandat 2793.970 kb on - strandat 2793.970 kb on - strandat 2794.028 kb on + strandat 2794.028 kb on + strandat 2794.603 kb on - strand, within BPHYT_RS12235at 2794.630 kb on - strand, within BPHYT_RS12235at 2794.987 kb on + strand, within BPHYT_RS12235at 2794.989 kb on + strand, within BPHYT_RS12235at 2794.990 kb on - strand, within BPHYT_RS12235at 2794.990 kb on - strand, within BPHYT_RS12235at 2794.990 kb on - strand, within BPHYT_RS12235at 2795.323 kb on - strandat 2795.436 kb on + strandat 2795.437 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,792,974 - BPHYT_RS12225 0.66 -1.3
2,793,321 + -0.1
2,793,322 - +0.2
2,793,604 + BPHYT_RS12230 0.21 -3.6
2,793,693 + BPHYT_RS12230 0.53 -0.3
2,793,873 + +1.2
2,793,894 - -0.4
2,793,898 + -1.0
2,793,898 + +0.6
2,793,915 + -0.7
2,793,915 + -1.8
2,793,970 - -0.7
2,793,970 - +1.4
2,793,970 - -0.0
2,794,028 + +0.2
2,794,028 + -2.5
2,794,603 - BPHYT_RS12235 0.46 -1.9
2,794,630 - BPHYT_RS12235 0.48 +0.8
2,794,987 + BPHYT_RS12235 0.74 -2.6
2,794,989 + BPHYT_RS12235 0.74 +0.8
2,794,990 - BPHYT_RS12235 0.74 +0.6
2,794,990 - BPHYT_RS12235 0.74 +0.5
2,794,990 - BPHYT_RS12235 0.74 -0.0
2,795,323 - -1.4
2,795,436 + +1.5
2,795,437 - -1.1

Or see this region's nucleotide sequence