Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10960

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10955 and BPHYT_RS10960 are separated by 592 nucleotidesBPHYT_RS10960 and BPHYT_RS10965 are separated by 53 nucleotidesBPHYT_RS10965 and BPHYT_RS10970 are separated by 78 nucleotidesBPHYT_RS10970 and BPHYT_RS10975 are separated by 57 nucleotides BPHYT_RS10955: BPHYT_RS10955 - dioxygenase, at 2,492,643 to 2,493,575 _RS10955 BPHYT_RS10960: BPHYT_RS10960 - hypothetical protein, at 2,494,168 to 2,494,281 _RS10960 BPHYT_RS10965: BPHYT_RS10965 - hypothetical protein, at 2,494,335 to 2,494,724 _RS10965 BPHYT_RS10970: BPHYT_RS10970 - hypothetical protein, at 2,494,803 to 2,495,051 _RS10970 BPHYT_RS10975: BPHYT_RS10975 - hypothetical protein, at 2,495,109 to 2,495,351 _RS10975 Position (kb) 2494 2495Strain fitness (log2 ratio) -2 -1 0 1at 2493.311 kb on - strand, within BPHYT_RS10955at 2493.579 kb on - strandat 2493.579 kb on - strandat 2494.121 kb on - strandat 2494.133 kb on + strandat 2494.317 kb on - strandat 2494.557 kb on + strand, within BPHYT_RS10965at 2495.160 kb on - strand, within BPHYT_RS10975

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,493,311 - BPHYT_RS10955 0.72 +0.3
2,493,579 - -2.3
2,493,579 - +0.5
2,494,121 - +0.6
2,494,133 + -0.8
2,494,317 - +0.1
2,494,557 + BPHYT_RS10965 0.57 +0.3
2,495,160 - BPHYT_RS10975 0.21 +1.1

Or see this region's nucleotide sequence