Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10685 and BPHYT_RS10690 are separated by 106 nucleotides BPHYT_RS10690 and BPHYT_RS10695 overlap by 4 nucleotides BPHYT_RS10695 and BPHYT_RS10700 are separated by 32 nucleotides BPHYT_RS10700 and BPHYT_RS10705 are separated by 40 nucleotides
BPHYT_RS10685: BPHYT_RS10685 - LysR family transcriptional regulator, at 2,432,668 to 2,433,585
_RS10685
BPHYT_RS10690: BPHYT_RS10690 - Rieske (2Fe-2S) protein, at 2,433,692 to 2,434,975
_RS10690
BPHYT_RS10695: BPHYT_RS10695 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 2,434,972 to 2,435,472
_RS10695
BPHYT_RS10700: BPHYT_RS10700 - 4-oxalocrotonate tautomerase, at 2,435,505 to 2,435,888
_RS10700
BPHYT_RS10705: BPHYT_RS10705 - MFS transporter, at 2,435,929 to 2,437,245
_RS10705
Position (kb)
2433
2434
2435 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2433.071 kb on + strand, within BPHYT_RS10685 at 2433.071 kb on + strand, within BPHYT_RS10685 at 2433.196 kb on - strand at 2433.252 kb on - strand, within BPHYT_RS10685 at 2433.707 kb on + strand at 2434.035 kb on - strand, within BPHYT_RS10690 at 2434.137 kb on - strand, within BPHYT_RS10690 at 2435.108 kb on - strand, within BPHYT_RS10695 at 2435.510 kb on + strand at 2435.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,433,071 + BPHYT_RS10685 0.44 +1.0 2,433,071 + BPHYT_RS10685 0.44 -3.3 2,433,196 - +2.4 2,433,252 - BPHYT_RS10685 0.64 -1.1 2,433,707 + +0.3 2,434,035 - BPHYT_RS10690 0.27 -0.8 2,434,137 - BPHYT_RS10690 0.35 -4.0 2,435,108 - BPHYT_RS10695 0.27 +0.2 2,435,510 + -0.0 2,435,511 - +1.4
Or see this region's nucleotide sequence