Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09825

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09815 and BPHYT_RS09820 are separated by 121 nucleotidesBPHYT_RS09820 and BPHYT_RS09825 are separated by 400 nucleotidesBPHYT_RS09825 and BPHYT_RS09835 are separated by 342 nucleotides BPHYT_RS09815: BPHYT_RS09815 - malate synthase, at 2,228,975 to 2,231,149 _RS09815 BPHYT_RS09820: BPHYT_RS09820 - hypothetical protein, at 2,231,271 to 2,231,675 _RS09820 BPHYT_RS09825: BPHYT_RS09825 - Fe-S protein, at 2,232,076 to 2,232,951 _RS09825 BPHYT_RS09835: BPHYT_RS09835 - uroporphyrin-III C-methyltransferase, at 2,233,294 to 2,234,109 _RS09835 Position (kb) 2232 2233Strain fitness (log2 ratio) -2 -1 0 1at 2231.678 kb on + strandat 2231.701 kb on + strandat 2232.095 kb on + strandat 2232.096 kb on - strandat 2232.226 kb on + strand, within BPHYT_RS09825at 2232.487 kb on + strand, within BPHYT_RS09825at 2232.670 kb on + strand, within BPHYT_RS09825at 2233.052 kb on - strandat 2233.147 kb on - strandat 2233.211 kb on - strandat 2233.599 kb on + strand, within BPHYT_RS09835

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,231,678 + -0.1
2,231,701 + -0.3
2,232,095 + +1.1
2,232,096 - -0.5
2,232,226 + BPHYT_RS09825 0.17 +0.3
2,232,487 + BPHYT_RS09825 0.47 +0.7
2,232,670 + BPHYT_RS09825 0.68 -2.3
2,233,052 - +0.0
2,233,147 - -2.4
2,233,211 - +0.3
2,233,599 + BPHYT_RS09835 0.37 +0.5

Or see this region's nucleotide sequence