Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09090 and BPHYT_RS09095 are separated by 148 nucleotides BPHYT_RS09095 and BPHYT_RS09100 are separated by 224 nucleotides BPHYT_RS09100 and BPHYT_RS09105 overlap by 4 nucleotides
BPHYT_RS09090: BPHYT_RS09090 - ABC transporter permease, at 2,056,901 to 2,057,725
_RS09090
BPHYT_RS09095: BPHYT_RS09095 - NADPH:quinone oxidoreductase, at 2,057,874 to 2,058,848
_RS09095
BPHYT_RS09100: BPHYT_RS09100 - stationary phase survival protein SurE, at 2,059,073 to 2,059,831
_RS09100
BPHYT_RS09105: BPHYT_RS09105 - protein-L-isoaspartate O-methyltransferase, at 2,059,828 to 2,060,880
_RS09105
Position (kb)
2057
2058
2059 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2057.112 kb on - strand, within BPHYT_RS09090 at 2057.235 kb on - strand, within BPHYT_RS09090 at 2057.366 kb on + strand, within BPHYT_RS09090 at 2057.494 kb on + strand, within BPHYT_RS09090 at 2057.495 kb on - strand, within BPHYT_RS09090 at 2057.537 kb on + strand, within BPHYT_RS09090 at 2057.538 kb on - strand, within BPHYT_RS09090 at 2057.602 kb on + strand, within BPHYT_RS09090 at 2057.603 kb on - strand, within BPHYT_RS09090 at 2057.603 kb on - strand, within BPHYT_RS09090 at 2058.211 kb on + strand, within BPHYT_RS09095 at 2058.277 kb on + strand, within BPHYT_RS09095 at 2058.278 kb on - strand, within BPHYT_RS09095 at 2058.698 kb on - strand, within BPHYT_RS09095 at 2058.880 kb on + strand at 2058.881 kb on - strand at 2059.010 kb on + strand at 2059.010 kb on + strand at 2059.256 kb on + strand, within BPHYT_RS09100 at 2059.256 kb on + strand, within BPHYT_RS09100 at 2059.257 kb on - strand, within BPHYT_RS09100 at 2059.315 kb on + strand, within BPHYT_RS09100 at 2059.316 kb on - strand, within BPHYT_RS09100 at 2059.316 kb on - strand, within BPHYT_RS09100
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,057,112 - BPHYT_RS09090 0.26 -1.8 2,057,235 - BPHYT_RS09090 0.40 -0.8 2,057,366 + BPHYT_RS09090 0.56 -0.6 2,057,494 + BPHYT_RS09090 0.72 -0.8 2,057,495 - BPHYT_RS09090 0.72 +0.2 2,057,537 + BPHYT_RS09090 0.77 -0.1 2,057,538 - BPHYT_RS09090 0.77 -1.5 2,057,602 + BPHYT_RS09090 0.85 -0.5 2,057,603 - BPHYT_RS09090 0.85 +2.2 2,057,603 - BPHYT_RS09090 0.85 -2.5 2,058,211 + BPHYT_RS09095 0.35 +1.4 2,058,277 + BPHYT_RS09095 0.41 +1.9 2,058,278 - BPHYT_RS09095 0.41 +0.4 2,058,698 - BPHYT_RS09095 0.85 -0.6 2,058,880 + +0.9 2,058,881 - -0.2 2,059,010 + -1.0 2,059,010 + +3.6 2,059,256 + BPHYT_RS09100 0.24 +0.1 2,059,256 + BPHYT_RS09100 0.24 -1.2 2,059,257 - BPHYT_RS09100 0.24 -0.6 2,059,315 + BPHYT_RS09100 0.32 -0.4 2,059,316 - BPHYT_RS09100 0.32 +0.6 2,059,316 - BPHYT_RS09100 0.32 -1.0
Or see this region's nucleotide sequence