Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08990 and BPHYT_RS08995 are separated by 145 nucleotides BPHYT_RS08995 and BPHYT_RS09000 are separated by 219 nucleotides BPHYT_RS09000 and BPHYT_RS09005 are separated by 322 nucleotides BPHYT_RS09005 and BPHYT_RS09010 overlap by 49 nucleotides
BPHYT_RS08990: BPHYT_RS08990 - protein disaggregation chaperone, at 2,033,979 to 2,036,576
_RS08990
BPHYT_RS08995: BPHYT_RS08995 - hypothetical protein, at 2,036,722 to 2,037,150
_RS08995
BPHYT_RS09000: BPHYT_RS09000 - competence protein ComE, at 2,037,370 to 2,037,960
_RS09000
BPHYT_RS09005: BPHYT_RS09005 - MerR family transcriptional regulator, at 2,038,283 to 2,038,777
_RS09005
BPHYT_RS09010: BPHYT_RS09010 - 4-carboxymuconolactone decarboxylase, at 2,038,729 to 2,039,187
_RS09010
Position (kb)
2037
2038 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2036.929 kb on + strand, within BPHYT_RS08995 at 2036.930 kb on - strand, within BPHYT_RS08995 at 2037.303 kb on + strand at 2037.304 kb on - strand at 2037.365 kb on + strand at 2037.610 kb on - strand, within BPHYT_RS09000 at 2037.943 kb on + strand at 2037.943 kb on + strand at 2037.944 kb on - strand at 2037.944 kb on - strand at 2037.944 kb on - strand at 2037.944 kb on - strand at 2038.055 kb on - strand at 2038.060 kb on + strand at 2038.061 kb on - strand at 2038.166 kb on - strand at 2038.203 kb on + strand at 2038.248 kb on + strand at 2038.291 kb on + strand at 2038.348 kb on + strand, within BPHYT_RS09005 at 2038.348 kb on + strand, within BPHYT_RS09005 at 2038.349 kb on - strand, within BPHYT_RS09005 at 2038.361 kb on + strand, within BPHYT_RS09005 at 2038.418 kb on + strand, within BPHYT_RS09005 at 2038.634 kb on + strand, within BPHYT_RS09005 at 2038.637 kb on + strand, within BPHYT_RS09005 at 2038.638 kb on - strand, within BPHYT_RS09005 at 2038.638 kb on - strand, within BPHYT_RS09005 at 2038.850 kb on + strand, within BPHYT_RS09010
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,036,929 + BPHYT_RS08995 0.48 -0.1 2,036,930 - BPHYT_RS08995 0.48 -0.6 2,037,303 + -0.9 2,037,304 - +1.6 2,037,365 + +0.6 2,037,610 - BPHYT_RS09000 0.41 -2.4 2,037,943 + -0.3 2,037,943 + -2.0 2,037,944 - +1.7 2,037,944 - +2.7 2,037,944 - +0.1 2,037,944 - -0.5 2,038,055 - +3.4 2,038,060 + +1.3 2,038,061 - -0.2 2,038,166 - +2.2 2,038,203 + +0.9 2,038,248 + -2.0 2,038,291 + +0.4 2,038,348 + BPHYT_RS09005 0.13 -0.1 2,038,348 + BPHYT_RS09005 0.13 -2.5 2,038,349 - BPHYT_RS09005 0.13 +1.2 2,038,361 + BPHYT_RS09005 0.16 +0.5 2,038,418 + BPHYT_RS09005 0.27 +0.2 2,038,634 + BPHYT_RS09005 0.71 +0.4 2,038,637 + BPHYT_RS09005 0.72 +0.1 2,038,638 - BPHYT_RS09005 0.72 -0.5 2,038,638 - BPHYT_RS09005 0.72 -0.4 2,038,850 + BPHYT_RS09010 0.26 +0.7
Or see this region's nucleotide sequence