Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08030 and BPHYT_RS08035 are separated by 406 nucleotides BPHYT_RS08035 and BPHYT_RS08040 are separated by 393 nucleotides
BPHYT_RS08030: BPHYT_RS08030 - transposase, at 1,830,419 to 1,830,820
_RS08030
BPHYT_RS08035: BPHYT_RS08035 - LysR family transcriptional regulator, at 1,831,227 to 1,832,129
_RS08035
BPHYT_RS08040: BPHYT_RS08040 - aspartate aminotransferase, at 1,832,523 to 1,833,749
_RS08040
Position (kb)
1831
1832
1833 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1830.374 kb on + strand at 1830.376 kb on - strand at 1830.376 kb on - strand at 1830.575 kb on + strand, within BPHYT_RS08030 at 1830.575 kb on + strand, within BPHYT_RS08030 at 1830.576 kb on - strand, within BPHYT_RS08030 at 1830.576 kb on - strand, within BPHYT_RS08030 at 1830.680 kb on + strand, within BPHYT_RS08030 at 1830.839 kb on + strand at 1830.839 kb on + strand at 1830.925 kb on + strand at 1831.048 kb on - strand at 1831.048 kb on - strand at 1831.250 kb on - strand at 1831.403 kb on + strand, within BPHYT_RS08035 at 1831.559 kb on - strand, within BPHYT_RS08035 at 1831.559 kb on - strand, within BPHYT_RS08035 at 1831.587 kb on + strand, within BPHYT_RS08035 at 1831.587 kb on + strand, within BPHYT_RS08035 at 1831.589 kb on + strand, within BPHYT_RS08035 at 1831.592 kb on - strand, within BPHYT_RS08035 at 1832.127 kb on + strand at 1832.127 kb on + strand at 1832.242 kb on - strand at 1832.336 kb on + strand at 1832.337 kb on - strand at 1832.337 kb on - strand at 1832.466 kb on + strand at 1832.466 kb on + strand at 1832.472 kb on - strand at 1832.472 kb on - strand at 1832.730 kb on + strand, within BPHYT_RS08040 at 1832.730 kb on + strand, within BPHYT_RS08040 at 1832.730 kb on + strand, within BPHYT_RS08040
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,830,374 + -0.9 1,830,376 - -0.2 1,830,376 - -0.2 1,830,575 + BPHYT_RS08030 0.39 -0.4 1,830,575 + BPHYT_RS08030 0.39 +2.5 1,830,576 - BPHYT_RS08030 0.39 -1.5 1,830,576 - BPHYT_RS08030 0.39 -0.2 1,830,680 + BPHYT_RS08030 0.65 +0.6 1,830,839 + -1.7 1,830,839 + -0.8 1,830,925 + +2.0 1,831,048 - -0.3 1,831,048 - -2.7 1,831,250 - -0.9 1,831,403 + BPHYT_RS08035 0.19 -1.8 1,831,559 - BPHYT_RS08035 0.37 +0.1 1,831,559 - BPHYT_RS08035 0.37 -2.1 1,831,587 + BPHYT_RS08035 0.40 -0.5 1,831,587 + BPHYT_RS08035 0.40 -0.8 1,831,589 + BPHYT_RS08035 0.40 +0.1 1,831,592 - BPHYT_RS08035 0.40 -1.2 1,832,127 + +0.1 1,832,127 + -1.5 1,832,242 - -0.4 1,832,336 + -1.2 1,832,337 - +0.5 1,832,337 - +1.9 1,832,466 + +0.1 1,832,466 + +0.8 1,832,472 - -2.2 1,832,472 - -1.4 1,832,730 + BPHYT_RS08040 0.17 +1.0 1,832,730 + BPHYT_RS08040 0.17 +0.2 1,832,730 + BPHYT_RS08040 0.17 +0.2
Or see this region's nucleotide sequence