Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05765 and BPHYT_RS05770 are separated by 145 nucleotides BPHYT_RS05770 and BPHYT_RS05775 are separated by 81 nucleotides BPHYT_RS05775 and BPHYT_RS05780 overlap by 4 nucleotides BPHYT_RS05780 and BPHYT_RS05785 overlap by 4 nucleotides
BPHYT_RS05765: BPHYT_RS05765 - portal protein, at 1,311,525 to 1,312,994
_RS05765
BPHYT_RS05770: BPHYT_RS05770 - peptidase U35, at 1,313,140 to 1,315,152
_RS05770
BPHYT_RS05775: BPHYT_RS05775 - hypothetical protein, at 1,315,234 to 1,315,563
_RS05775
BPHYT_RS05780: BPHYT_RS05780 - hypothetical protein, at 1,315,560 to 1,315,871
_RS05780
BPHYT_RS05785: BPHYT_RS05785 - hypothetical protein, at 1,315,868 to 1,316,299
_RS05785
Position (kb)
1313
1314
1315
1316 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1312.281 kb on + strand, within BPHYT_RS05765 at 1312.501 kb on + strand, within BPHYT_RS05765 at 1312.546 kb on - strand, within BPHYT_RS05765 at 1312.992 kb on + strand at 1312.992 kb on + strand at 1313.004 kb on + strand at 1313.005 kb on - strand at 1313.005 kb on - strand at 1313.005 kb on - strand at 1313.016 kb on + strand at 1313.263 kb on - strand at 1313.263 kb on - strand at 1313.739 kb on + strand, within BPHYT_RS05770 at 1313.889 kb on + strand, within BPHYT_RS05770 at 1314.346 kb on + strand, within BPHYT_RS05770 at 1314.473 kb on - strand, within BPHYT_RS05770 at 1314.478 kb on + strand, within BPHYT_RS05770 at 1314.599 kb on - strand, within BPHYT_RS05770 at 1314.640 kb on + strand, within BPHYT_RS05770 at 1314.641 kb on - strand, within BPHYT_RS05770 at 1314.641 kb on - strand, within BPHYT_RS05770 at 1314.641 kb on - strand, within BPHYT_RS05770 at 1314.739 kb on - strand, within BPHYT_RS05770 at 1314.875 kb on - strand, within BPHYT_RS05770 at 1314.875 kb on - strand, within BPHYT_RS05770 at 1315.009 kb on + strand at 1315.040 kb on - strand at 1315.150 kb on + strand at 1315.150 kb on + strand at 1315.151 kb on - strand at 1315.376 kb on - strand, within BPHYT_RS05775 at 1315.393 kb on + strand, within BPHYT_RS05775 at 1315.722 kb on + strand, within BPHYT_RS05780 at 1315.722 kb on + strand, within BPHYT_RS05780 at 1315.735 kb on - strand, within BPHYT_RS05780
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,312,281 + BPHYT_RS05765 0.51 +0.8 1,312,501 + BPHYT_RS05765 0.66 -2.0 1,312,546 - BPHYT_RS05765 0.69 +1.1 1,312,992 + -2.9 1,312,992 + +2.3 1,313,004 + -1.1 1,313,005 - +0.4 1,313,005 - -0.6 1,313,005 - -0.9 1,313,016 + +0.5 1,313,263 - -0.3 1,313,263 - -0.8 1,313,739 + BPHYT_RS05770 0.30 -1.2 1,313,889 + BPHYT_RS05770 0.37 -1.3 1,314,346 + BPHYT_RS05770 0.60 -0.6 1,314,473 - BPHYT_RS05770 0.66 -0.5 1,314,478 + BPHYT_RS05770 0.66 +0.7 1,314,599 - BPHYT_RS05770 0.72 -1.5 1,314,640 + BPHYT_RS05770 0.75 -0.0 1,314,641 - BPHYT_RS05770 0.75 -2.3 1,314,641 - BPHYT_RS05770 0.75 -0.7 1,314,641 - BPHYT_RS05770 0.75 -1.0 1,314,739 - BPHYT_RS05770 0.79 -0.2 1,314,875 - BPHYT_RS05770 0.86 -2.4 1,314,875 - BPHYT_RS05770 0.86 +0.7 1,315,009 + +2.6 1,315,040 - -1.3 1,315,150 + +0.0 1,315,150 + +1.2 1,315,151 - -0.1 1,315,376 - BPHYT_RS05775 0.43 -0.5 1,315,393 + BPHYT_RS05775 0.48 -0.1 1,315,722 + BPHYT_RS05780 0.52 -0.1 1,315,722 + BPHYT_RS05780 0.52 -0.5 1,315,735 - BPHYT_RS05780 0.56 -1.1
Or see this region's nucleotide sequence