Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03990 and BPHYT_RS03995 are separated by 49 nucleotides BPHYT_RS03995 and BPHYT_RS04000 are separated by 83 nucleotides
BPHYT_RS03990: BPHYT_RS03990 - sugar ABC transporter substrate-binding protein, at 907,781 to 908,920
_RS03990
BPHYT_RS03995: BPHYT_RS03995 - protein tyrosine kinase, at 908,970 to 911,204
_RS03995
BPHYT_RS04000: BPHYT_RS04000 - GDP-mannose 4,6-dehydratase, at 911,288 to 912,406
_RS04000
Position (kb) 908
909
910
911
912 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 908.211 kb on + strand, within BPHYT_RS03990 at 908.212 kb on - strand, within BPHYT_RS03990 at 908.255 kb on + strand, within BPHYT_RS03990 at 908.333 kb on - strand, within BPHYT_RS03990 at 908.582 kb on + strand, within BPHYT_RS03990 at 908.582 kb on + strand, within BPHYT_RS03990 at 909.036 kb on + strand at 909.111 kb on + strand at 909.160 kb on + strand at 909.160 kb on + strand at 909.919 kb on - strand, within BPHYT_RS03995 at 910.207 kb on - strand, within BPHYT_RS03995 at 910.398 kb on + strand, within BPHYT_RS03995 at 911.011 kb on - strand at 911.296 kb on + strand at 911.339 kb on + strand at 911.542 kb on + strand, within BPHYT_RS04000 at 911.675 kb on + strand, within BPHYT_RS04000 at 911.923 kb on + strand, within BPHYT_RS04000 at 911.940 kb on - strand, within BPHYT_RS04000 at 912.036 kb on - strand, within BPHYT_RS04000
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 908,211 + BPHYT_RS03990 0.38 +0.1 908,212 - BPHYT_RS03990 0.38 +0.1 908,255 + BPHYT_RS03990 0.42 -1.8 908,333 - BPHYT_RS03990 0.48 -2.5 908,582 + BPHYT_RS03990 0.70 -1.2 908,582 + BPHYT_RS03990 0.70 -0.6 909,036 + +0.1 909,111 + -1.2 909,160 + -2.0 909,160 + +1.9 909,919 - BPHYT_RS03995 0.42 -0.8 910,207 - BPHYT_RS03995 0.55 -2.3 910,398 + BPHYT_RS03995 0.64 +0.4 911,011 - +1.5 911,296 + +3.1 911,339 + -0.7 911,542 + BPHYT_RS04000 0.23 -1.1 911,675 + BPHYT_RS04000 0.35 -1.8 911,923 + BPHYT_RS04000 0.57 -0.1 911,940 - BPHYT_RS04000 0.58 -1.6 912,036 - BPHYT_RS04000 0.67 -0.5
Or see this region's nucleotide sequence