Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02760 and BPHYT_RS02765 are separated by 35 nucleotides BPHYT_RS02765 and BPHYT_RS02770 overlap by 11 nucleotides BPHYT_RS02770 and BPHYT_RS02775 are separated by 338 nucleotides
BPHYT_RS02760: BPHYT_RS02760 - cytochrome BD oxidase subunit II, at 619,886 to 621,022
_RS02760
BPHYT_RS02765: BPHYT_RS02765 - cytochrome BD oxidase subunit I, at 621,058 to 622,650
_RS02765
BPHYT_RS02770: BPHYT_RS02770 - membrane protein, at 622,640 to 622,900
_RS02770
BPHYT_RS02775: BPHYT_RS02775 - RNA polymerase sigma 70, at 623,239 to 624,174
_RS02775
Position (kb) 621
622
623 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 620.557 kb on - strand, within BPHYT_RS02760 at 620.677 kb on + strand, within BPHYT_RS02760 at 621.224 kb on + strand, within BPHYT_RS02765 at 622.163 kb on + strand, within BPHYT_RS02765 at 622.397 kb on + strand, within BPHYT_RS02765 at 622.526 kb on + strand at 622.526 kb on + strand at 622.526 kb on + strand at 622.527 kb on - strand at 622.726 kb on + strand, within BPHYT_RS02770 at 622.727 kb on - strand, within BPHYT_RS02770 at 622.727 kb on - strand, within BPHYT_RS02770 at 622.727 kb on - strand, within BPHYT_RS02770 at 622.778 kb on + strand, within BPHYT_RS02770 at 622.892 kb on + strand at 623.054 kb on + strand at 623.055 kb on - strand at 623.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 620,557 - BPHYT_RS02760 0.59 +0.4 620,677 + BPHYT_RS02760 0.70 -1.2 621,224 + BPHYT_RS02765 0.10 +0.2 622,163 + BPHYT_RS02765 0.69 -0.9 622,397 + BPHYT_RS02765 0.84 +0.2 622,526 + -0.4 622,526 + +1.2 622,526 + +2.0 622,527 - +3.8 622,726 + BPHYT_RS02770 0.33 +0.9 622,727 - BPHYT_RS02770 0.33 -2.4 622,727 - BPHYT_RS02770 0.33 +1.5 622,727 - BPHYT_RS02770 0.33 +2.7 622,778 + BPHYT_RS02770 0.53 -1.7 622,892 + +2.2 623,054 + +0.6 623,055 - +1.9 623,055 - -1.2
Or see this region's nucleotide sequence