Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02085 and BPHYT_RS02090 are separated by 224 nucleotides BPHYT_RS02090 and BPHYT_RS02095 are separated by 283 nucleotides
BPHYT_RS02085: BPHYT_RS02085 - methylmalonyl-CoA mutase, at 477,797 to 481,693
_RS02085
BPHYT_RS02090: BPHYT_RS02090 - 2-nitropropane dioxygenase, at 481,918 to 482,877
_RS02090
BPHYT_RS02095: BPHYT_RS02095 - alpha/beta hydrolase, at 483,161 to 484,051
_RS02095
Position (kb) 481
482
483 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 480.995 kb on + strand, within BPHYT_RS02085 at 480.996 kb on - strand, within BPHYT_RS02085 at 481.373 kb on + strand at 481.373 kb on + strand at 481.373 kb on + strand at 481.373 kb on + strand at 481.374 kb on - strand at 481.374 kb on - strand at 481.374 kb on - strand at 481.420 kb on + strand at 481.420 kb on + strand at 481.687 kb on + strand at 481.688 kb on - strand at 481.691 kb on + strand at 481.691 kb on + strand at 481.692 kb on - strand at 481.692 kb on - strand at 481.953 kb on + strand at 481.954 kb on - strand at 481.954 kb on - strand at 481.955 kb on + strand at 482.144 kb on + strand, within BPHYT_RS02090 at 482.144 kb on + strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.175 kb on + strand, within BPHYT_RS02090 at 482.178 kb on - strand, within BPHYT_RS02090 at 482.220 kb on - strand, within BPHYT_RS02090 at 482.270 kb on + strand, within BPHYT_RS02090 at 482.270 kb on + strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.357 kb on - strand, within BPHYT_RS02090 at 482.357 kb on - strand, within BPHYT_RS02090 at 482.510 kb on + strand, within BPHYT_RS02090 at 482.510 kb on + strand, within BPHYT_RS02090 at 482.511 kb on - strand, within BPHYT_RS02090 at 482.514 kb on + strand, within BPHYT_RS02090 at 482.515 kb on - strand, within BPHYT_RS02090 at 482.877 kb on + strand at 482.877 kb on + strand at 482.877 kb on + strand at 483.078 kb on + strand at 483.079 kb on - strand at 483.079 kb on - strand at 483.079 kb on - strand at 483.348 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.674 kb on + strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand at 483.794 kb on + strand, within BPHYT_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 480,995 + BPHYT_RS02085 0.82 +1.9 480,996 - BPHYT_RS02085 0.82 -1.1 481,373 + -0.9 481,373 + +0.1 481,373 + +1.8 481,373 + +0.2 481,374 - +0.5 481,374 - -0.3 481,374 - -1.8 481,420 + +0.6 481,420 + +0.0 481,687 + -1.0 481,688 - -0.4 481,691 + +0.8 481,691 + +0.3 481,692 - -1.5 481,692 - -0.4 481,953 + +0.2 481,954 - -2.7 481,954 - +0.4 481,955 + -1.9 482,144 + BPHYT_RS02090 0.24 -0.1 482,144 + BPHYT_RS02090 0.24 +0.0 482,145 - BPHYT_RS02090 0.24 +1.5 482,145 - BPHYT_RS02090 0.24 +0.5 482,145 - BPHYT_RS02090 0.24 +1.1 482,175 + BPHYT_RS02090 0.27 -1.1 482,178 - BPHYT_RS02090 0.27 -1.3 482,220 - BPHYT_RS02090 0.31 -0.3 482,270 + BPHYT_RS02090 0.37 -0.3 482,270 + BPHYT_RS02090 0.37 +0.4 482,271 - BPHYT_RS02090 0.37 +0.7 482,271 - BPHYT_RS02090 0.37 +0.2 482,271 - BPHYT_RS02090 0.37 +0.9 482,357 - BPHYT_RS02090 0.46 -0.3 482,357 - BPHYT_RS02090 0.46 +0.9 482,510 + BPHYT_RS02090 0.62 +1.4 482,510 + BPHYT_RS02090 0.62 -1.1 482,511 - BPHYT_RS02090 0.62 +0.3 482,514 + BPHYT_RS02090 0.62 +0.4 482,515 - BPHYT_RS02090 0.62 -0.0 482,877 + +0.4 482,877 + +0.4 482,877 + +0.3 483,078 + -2.8 483,079 - +0.1 483,079 - -0.8 483,079 - -0.1 483,348 - BPHYT_RS02095 0.21 -0.6 483,570 - BPHYT_RS02095 0.46 -0.3 483,570 - BPHYT_RS02095 0.46 +0.0 483,570 - BPHYT_RS02095 0.46 -0.9 483,674 + BPHYT_RS02095 0.58 +0.0 483,675 - BPHYT_RS02095 0.58 -2.4 483,675 - BPHYT_RS02095 0.58 +0.4 483,675 - BPHYT_RS02095 0.58 -0.3 483,675 - -0.8 483,794 + BPHYT_RS02095 0.71 -0.9
Or see this region's nucleotide sequence