Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02000 and BPHYT_RS02005 are separated by 373 nucleotides BPHYT_RS02005 and BPHYT_RS02010 are separated by 44 nucleotides BPHYT_RS02010 and BPHYT_RS02015 are separated by 95 nucleotides
BPHYT_RS02000: BPHYT_RS02000 - sodium:proton antiporter, at 452,811 to 454,466
_RS02000
BPHYT_RS02005: BPHYT_RS02005 - diguanylate phosphodiesterase, at 454,840 to 456,108
_RS02005
BPHYT_RS02010: BPHYT_RS02010 - copper homeostasis protein CutC, at 456,153 to 456,848
_RS02010
BPHYT_RS02015: BPHYT_RS02015 - phenylpyruvate ferredoxin oxidoreductase (EC 1.2.7.8) <small>(from data)</small>, at 456,944 to 460,531
_RS02015
Position (kb) 454
455
456
457 Strain fitness (log2 ratio) -2
-1
0
1 at 454.029 kb on - strand, within BPHYT_RS02000 at 454.446 kb on + strand at 454.447 kb on - strand at 454.451 kb on + strand at 454.484 kb on + strand at 454.484 kb on + strand at 454.485 kb on - strand at 454.486 kb on + strand at 454.486 kb on + strand at 454.486 kb on + strand at 454.487 kb on - strand at 454.603 kb on + strand at 454.704 kb on + strand at 454.748 kb on + strand at 455.138 kb on + strand, within BPHYT_RS02005 at 455.201 kb on - strand, within BPHYT_RS02005 at 455.950 kb on + strand, within BPHYT_RS02005 at 456.153 kb on - strand at 456.355 kb on - strand, within BPHYT_RS02010 at 456.355 kb on - strand, within BPHYT_RS02010 at 456.370 kb on - strand, within BPHYT_RS02010 at 456.854 kb on + strand at 456.859 kb on - strand at 456.859 kb on - strand at 456.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 454,029 - BPHYT_RS02000 0.74 +0.4 454,446 + +0.9 454,447 - -0.6 454,451 + +0.4 454,484 + -0.3 454,484 + -1.7 454,485 - -2.1 454,486 + -1.0 454,486 + -1.1 454,486 + -0.6 454,487 - +0.4 454,603 + +0.4 454,704 + +0.7 454,748 + +0.2 455,138 + BPHYT_RS02005 0.23 -1.1 455,201 - BPHYT_RS02005 0.28 -1.1 455,950 + BPHYT_RS02005 0.87 -1.3 456,153 - +0.6 456,355 - BPHYT_RS02010 0.29 -0.9 456,355 - BPHYT_RS02010 0.29 +0.8 456,370 - BPHYT_RS02010 0.31 -1.6 456,854 + +1.0 456,859 - -0.1 456,859 - +1.4 456,859 - -1.3
Or see this region's nucleotide sequence