Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00745 and BPHYT_RS00750 overlap by 4 nucleotides BPHYT_RS00750 and BPHYT_RS00755 are separated by 526 nucleotides BPHYT_RS00755 and BPHYT_RS00760 overlap by 8 nucleotides BPHYT_RS00760 and BPHYT_RS00765 overlap by 4 nucleotides
BPHYT_RS00745: BPHYT_RS00745 - transcriptional regulator, at 171,763 to 172,167
_RS00745
BPHYT_RS00750: BPHYT_RS00750 - SpoVT/AbrB domain-containing protein, at 172,164 to 172,436
_RS00750
BPHYT_RS00755: BPHYT_RS00755 - cobyric acid synthase CobQ, at 172,963 to 173,835
_RS00755
BPHYT_RS00760: BPHYT_RS00760 - hypothetical protein, at 173,828 to 174,412
_RS00760
BPHYT_RS00765: BPHYT_RS00765 - lytic transglycosylase, at 174,409 to 175,086
_RS00765
Position (kb)
172
173
174 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 172.158 kb on - strand at 172.171 kb on - strand at 172.171 kb on - strand at 172.171 kb on - strand at 172.171 kb on - strand at 172.318 kb on + strand, within BPHYT_RS00750 at 172.319 kb on - strand, within BPHYT_RS00750 at 172.482 kb on + strand at 172.504 kb on + strand at 172.505 kb on - strand at 172.505 kb on - strand at 172.507 kb on + strand at 172.508 kb on - strand at 172.508 kb on - strand at 172.508 kb on - strand at 172.508 kb on - strand at 172.695 kb on + strand at 172.695 kb on + strand at 172.696 kb on - strand at 172.703 kb on + strand at 172.754 kb on - strand at 172.754 kb on - strand at 172.830 kb on + strand at 172.830 kb on + strand at 172.830 kb on + strand at 173.069 kb on + strand, within BPHYT_RS00755 at 173.069 kb on + strand, within BPHYT_RS00755 at 173.069 kb on + strand, within BPHYT_RS00755 at 173.070 kb on - strand, within BPHYT_RS00755 at 173.070 kb on - strand, within BPHYT_RS00755 at 173.180 kb on + strand, within BPHYT_RS00755 at 173.372 kb on + strand, within BPHYT_RS00755 at 173.373 kb on - strand, within BPHYT_RS00755 at 173.446 kb on + strand, within BPHYT_RS00755 at 173.446 kb on + strand, within BPHYT_RS00755 at 173.447 kb on - strand, within BPHYT_RS00755 at 173.447 kb on - strand, within BPHYT_RS00755 at 173.643 kb on + strand, within BPHYT_RS00755 at 173.644 kb on - strand, within BPHYT_RS00755 at 174.023 kb on + strand, within BPHYT_RS00760 at 174.254 kb on + strand, within BPHYT_RS00760 at 174.254 kb on + strand, within BPHYT_RS00760 at 174.309 kb on - strand, within BPHYT_RS00760 at 174.336 kb on - strand, within BPHYT_RS00760 at 174.336 kb on - strand, within BPHYT_RS00760 at 174.515 kb on - strand, within BPHYT_RS00765 at 174.613 kb on + strand, within BPHYT_RS00765 at 174.614 kb on - strand, within BPHYT_RS00765 at 174.635 kb on + strand, within BPHYT_RS00765 at 174.806 kb on - strand, within BPHYT_RS00765 at 174.806 kb on - strand, within BPHYT_RS00765
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 172,158 - -2.8 172,171 - +1.0 172,171 - -0.1 172,171 - -1.3 172,171 - +1.3 172,318 + BPHYT_RS00750 0.56 +0.2 172,319 - BPHYT_RS00750 0.57 -0.2 172,482 + -1.5 172,504 + -1.4 172,505 - -2.3 172,505 - -1.1 172,507 + -0.7 172,508 - -1.3 172,508 - -0.8 172,508 - +0.3 172,508 - +0.6 172,695 + -0.2 172,695 + -0.3 172,696 - +1.1 172,703 + -0.2 172,754 - +1.2 172,754 - +0.6 172,830 + +0.3 172,830 + -1.2 172,830 + +0.4 173,069 + BPHYT_RS00755 0.12 -1.7 173,069 + BPHYT_RS00755 0.12 +0.0 173,069 + BPHYT_RS00755 0.12 -0.1 173,070 - BPHYT_RS00755 0.12 -0.3 173,070 - BPHYT_RS00755 0.12 +0.6 173,180 + BPHYT_RS00755 0.25 -1.1 173,372 + BPHYT_RS00755 0.47 -1.4 173,373 - BPHYT_RS00755 0.47 +0.1 173,446 + BPHYT_RS00755 0.55 -0.3 173,446 + BPHYT_RS00755 0.55 -0.5 173,447 - BPHYT_RS00755 0.55 +1.1 173,447 - BPHYT_RS00755 0.55 -0.6 173,643 + BPHYT_RS00755 0.78 +0.1 173,644 - BPHYT_RS00755 0.78 -0.8 174,023 + BPHYT_RS00760 0.33 +0.0 174,254 + BPHYT_RS00760 0.73 -0.5 174,254 + BPHYT_RS00760 0.73 +1.0 174,309 - BPHYT_RS00760 0.82 +0.6 174,336 - BPHYT_RS00760 0.87 -0.2 174,336 - BPHYT_RS00760 0.87 +1.3 174,515 - BPHYT_RS00765 0.16 -2.3 174,613 + BPHYT_RS00765 0.30 +0.1 174,614 - BPHYT_RS00765 0.30 +0.0 174,635 + BPHYT_RS00765 0.33 -0.6 174,806 - BPHYT_RS00765 0.59 -1.4 174,806 - BPHYT_RS00765 0.59 -0.7
Or see this region's nucleotide sequence