Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00630 and BPHYT_RS00635 overlap by 4 nucleotides BPHYT_RS00635 and BPHYT_RS00640 are separated by 55 nucleotides
BPHYT_RS00630: BPHYT_RS00630 - 3-oxoacyl-ACP synthase, at 149,904 to 150,965
_RS00630
BPHYT_RS00635: BPHYT_RS00635 - acetyltransferase, at 150,962 to 151,540
_RS00635
BPHYT_RS00640: BPHYT_RS00640 - acyl-CoA dehydrogenase, at 151,596 to 152,684
_RS00640
Position (kb) 150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 150.060 kb on + strand, within BPHYT_RS00630 at 150.276 kb on - strand, within BPHYT_RS00630 at 150.450 kb on + strand, within BPHYT_RS00630 at 150.450 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.618 kb on - strand, within BPHYT_RS00630 at 150.823 kb on + strand, within BPHYT_RS00630 at 150.920 kb on + strand at 150.921 kb on - strand at 151.020 kb on + strand, within BPHYT_RS00635 at 151.021 kb on - strand, within BPHYT_RS00635 at 151.021 kb on - strand, within BPHYT_RS00635 at 151.118 kb on + strand, within BPHYT_RS00635 at 151.119 kb on - strand, within BPHYT_RS00635 at 151.119 kb on - strand at 151.591 kb on - strand at 151.591 kb on - strand at 151.591 kb on - strand at 151.591 kb on - strand at 151.702 kb on + strand at 151.702 kb on + strand at 151.703 kb on - strand at 151.783 kb on - strand, within BPHYT_RS00640 at 151.833 kb on + strand, within BPHYT_RS00640 at 151.833 kb on + strand at 151.833 kb on + strand, within BPHYT_RS00640 at 151.834 kb on - strand, within BPHYT_RS00640 at 151.933 kb on + strand, within BPHYT_RS00640 at 151.990 kb on + strand, within BPHYT_RS00640 at 151.990 kb on + strand, within BPHYT_RS00640 at 151.991 kb on - strand, within BPHYT_RS00640 at 152.070 kb on - strand, within BPHYT_RS00640 at 152.099 kb on - strand, within BPHYT_RS00640 at 152.099 kb on - strand, within BPHYT_RS00640 at 152.144 kb on - strand, within BPHYT_RS00640 at 152.166 kb on + strand, within BPHYT_RS00640 at 152.309 kb on - strand, within BPHYT_RS00640 at 152.309 kb on - strand, within BPHYT_RS00640 at 152.318 kb on + strand, within BPHYT_RS00640 at 152.319 kb on - strand, within BPHYT_RS00640 at 152.351 kb on - strand, within BPHYT_RS00640
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 150,060 + BPHYT_RS00630 0.15 +2.8 150,276 - BPHYT_RS00630 0.35 -1.8 150,450 + BPHYT_RS00630 0.51 +0.5 150,450 + BPHYT_RS00630 0.51 -0.2 150,617 + BPHYT_RS00630 0.67 -0.0 150,617 + BPHYT_RS00630 0.67 -1.5 150,617 + BPHYT_RS00630 0.67 -1.8 150,617 + BPHYT_RS00630 0.67 -2.5 150,617 + BPHYT_RS00630 0.67 -2.2 150,618 - BPHYT_RS00630 0.67 -2.4 150,823 + BPHYT_RS00630 0.87 +0.4 150,920 + +0.6 150,921 - -0.2 151,020 + BPHYT_RS00635 0.10 -0.1 151,021 - BPHYT_RS00635 0.10 -0.6 151,021 - BPHYT_RS00635 0.10 -2.1 151,118 + BPHYT_RS00635 0.27 -1.0 151,119 - BPHYT_RS00635 0.27 -2.4 151,119 - +0.0 151,591 - +0.6 151,591 - -1.1 151,591 - -0.9 151,591 - -0.1 151,702 + -0.6 151,702 + +0.2 151,703 - +0.2 151,783 - BPHYT_RS00640 0.17 -0.4 151,833 + BPHYT_RS00640 0.22 -0.3 151,833 + +0.7 151,833 + BPHYT_RS00640 0.22 +0.7 151,834 - BPHYT_RS00640 0.22 +0.6 151,933 + BPHYT_RS00640 0.31 +0.2 151,990 + BPHYT_RS00640 0.36 -0.1 151,990 + BPHYT_RS00640 0.36 +1.3 151,991 - BPHYT_RS00640 0.36 -1.0 152,070 - BPHYT_RS00640 0.44 +1.3 152,099 - BPHYT_RS00640 0.46 -1.5 152,099 - BPHYT_RS00640 0.46 +1.0 152,144 - BPHYT_RS00640 0.50 -0.7 152,166 + BPHYT_RS00640 0.52 +3.6 152,309 - BPHYT_RS00640 0.65 -3.4 152,309 - BPHYT_RS00640 0.65 -0.2 152,318 + BPHYT_RS00640 0.66 +0.4 152,319 - BPHYT_RS00640 0.66 +0.4 152,351 - BPHYT_RS00640 0.69 +0.0
Or see this region's nucleotide sequence