Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36290 and BPHYT_RS36295 are separated by 43 nucleotides BPHYT_RS36295 and BPHYT_RS36300 are separated by 576 nucleotides BPHYT_RS36300 and BPHYT_RS36305 overlap by 32 nucleotides BPHYT_RS36305 and BPHYT_RS36310 are separated by 4 nucleotides BPHYT_RS36310 and BPHYT_RS36315 overlap by 17 nucleotides
BPHYT_RS36290: BPHYT_RS36290 - hypothetical protein, at 71,492 to 72,151
_RS36290
BPHYT_RS36295: BPHYT_RS36295 - hypothetical protein, at 72,195 to 72,563
_RS36295
BPHYT_RS36300: BPHYT_RS36300 - hypothetical protein, at 73,140 to 73,421
_RS36300
BPHYT_RS36305: BPHYT_RS36305 - hypothetical protein, at 73,390 to 73,812
_RS36305
BPHYT_RS36310: BPHYT_RS36310 - hypothetical protein, at 73,817 to 74,152
_RS36310
BPHYT_RS36315: BPHYT_RS36315 - CagE TrbE VirB component of type IV transporter system, at 74,136 to 76,685
_RS36315
Position (kb) 73
74 Strain fitness (log2 ratio) -1
0
1
2
3 at 72.170 kb on - strand at 72.170 kb on - strand at 72.223 kb on - strand at 72.256 kb on - strand, within BPHYT_RS36295 at 72.260 kb on - strand, within BPHYT_RS36295 at 72.260 kb on - strand, within BPHYT_RS36295 at 72.270 kb on - strand, within BPHYT_RS36295 at 72.270 kb on - strand, within BPHYT_RS36295 at 72.270 kb on - strand, within BPHYT_RS36295 at 72.270 kb on - strand at 72.270 kb on - strand, within BPHYT_RS36295 at 72.270 kb on - strand, within BPHYT_RS36295 at 72.274 kb on - strand, within BPHYT_RS36295 at 72.274 kb on - strand, within BPHYT_RS36295 at 72.350 kb on + strand, within BPHYT_RS36295 at 72.351 kb on - strand, within BPHYT_RS36295 at 72.352 kb on + strand, within BPHYT_RS36295 at 72.353 kb on - strand, within BPHYT_RS36295 at 72.353 kb on - strand, within BPHYT_RS36295 at 72.353 kb on - strand, within BPHYT_RS36295 at 72.445 kb on - strand at 72.462 kb on - strand, within BPHYT_RS36295 at 72.528 kb on - strand at 72.537 kb on - strand at 72.634 kb on + strand at 72.747 kb on - strand at 72.747 kb on - strand at 72.947 kb on - strand at 73.049 kb on - strand at 73.049 kb on - strand at 73.095 kb on - strand at 73.135 kb on - strand at 73.135 kb on - strand at 73.314 kb on - strand, within BPHYT_RS36300 at 73.362 kb on - strand, within BPHYT_RS36300 at 73.362 kb on - strand, within BPHYT_RS36300 at 73.362 kb on - strand, within BPHYT_RS36300 at 73.362 kb on - strand, within BPHYT_RS36300 at 73.362 kb on - strand, within BPHYT_RS36300 at 73.420 kb on - strand at 73.452 kb on + strand, within BPHYT_RS36305 at 73.501 kb on + strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.502 kb on - strand, within BPHYT_RS36305 at 73.600 kb on - strand, within BPHYT_RS36305 at 73.653 kb on - strand, within BPHYT_RS36305 at 73.817 kb on - strand at 73.884 kb on - strand, within BPHYT_RS36310 at 73.884 kb on - strand, within BPHYT_RS36310 at 73.888 kb on - strand, within BPHYT_RS36310 at 74.043 kb on - strand, within BPHYT_RS36310 at 74.043 kb on - strand, within BPHYT_RS36310 at 74.043 kb on - strand, within BPHYT_RS36310 at 74.043 kb on - strand, within BPHYT_RS36310 at 74.049 kb on - strand, within BPHYT_RS36310 at 74.136 kb on - strand at 74.136 kb on - strand at 74.136 kb on - strand at 74.136 kb on - strand at 74.136 kb on - strand at 74.146 kb on - strand at 74.178 kb on - strand at 74.184 kb on + strand at 74.184 kb on + strand at 74.199 kb on - strand at 74.199 kb on - strand at 74.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 72,170 - +0.1 72,170 - +0.1 72,223 - +0.5 72,256 - BPHYT_RS36295 0.17 +0.3 72,260 - BPHYT_RS36295 0.18 -0.9 72,260 - BPHYT_RS36295 0.18 +0.8 72,270 - BPHYT_RS36295 0.20 -0.3 72,270 - BPHYT_RS36295 0.20 +0.1 72,270 - BPHYT_RS36295 0.20 +0.2 72,270 - -0.6 72,270 - BPHYT_RS36295 0.20 -0.0 72,270 - BPHYT_RS36295 0.20 +0.1 72,274 - BPHYT_RS36295 0.21 -0.1 72,274 - BPHYT_RS36295 0.21 +0.2 72,350 + BPHYT_RS36295 0.42 -0.4 72,351 - BPHYT_RS36295 0.42 -0.0 72,352 + BPHYT_RS36295 0.43 -0.8 72,353 - BPHYT_RS36295 0.43 +0.3 72,353 - BPHYT_RS36295 0.43 +0.1 72,353 - BPHYT_RS36295 0.43 +0.2 72,445 - +3.5 72,462 - BPHYT_RS36295 0.72 -0.0 72,528 - +0.2 72,537 - +0.1 72,634 + -0.3 72,747 - -1.0 72,747 - +0.6 72,947 - +0.1 73,049 - +0.2 73,049 - -0.1 73,095 - -0.2 73,135 - +0.8 73,135 - -0.5 73,314 - BPHYT_RS36300 0.62 +1.9 73,362 - BPHYT_RS36300 0.79 -0.4 73,362 - BPHYT_RS36300 0.79 -0.8 73,362 - BPHYT_RS36300 0.79 -0.2 73,362 - BPHYT_RS36300 0.79 -0.7 73,362 - BPHYT_RS36300 0.79 +1.5 73,420 - +1.7 73,452 + BPHYT_RS36305 0.15 -0.4 73,501 + BPHYT_RS36305 0.26 -0.4 73,502 - BPHYT_RS36305 0.26 -0.4 73,502 - BPHYT_RS36305 0.26 +0.3 73,502 - BPHYT_RS36305 0.26 +0.4 73,502 - BPHYT_RS36305 0.26 +0.0 73,502 - BPHYT_RS36305 0.26 -0.3 73,502 - BPHYT_RS36305 0.26 +0.6 73,502 - BPHYT_RS36305 0.26 +0.6 73,600 - BPHYT_RS36305 0.50 +1.3 73,653 - BPHYT_RS36305 0.62 +0.3 73,817 - +0.3 73,884 - BPHYT_RS36310 0.20 +0.2 73,884 - BPHYT_RS36310 0.20 +0.9 73,888 - BPHYT_RS36310 0.21 +0.0 74,043 - BPHYT_RS36310 0.67 -0.4 74,043 - BPHYT_RS36310 0.67 +0.2 74,043 - BPHYT_RS36310 0.67 +0.2 74,043 - BPHYT_RS36310 0.67 +0.6 74,049 - BPHYT_RS36310 0.69 +0.4 74,136 - +0.2 74,136 - +0.3 74,136 - +0.1 74,136 - +0.6 74,136 - +1.0 74,146 - +0.3 74,178 - -0.0 74,184 + -0.5 74,184 + +1.3 74,199 - +0.2 74,199 - +3.0 74,250 - +0.4
Or see this region's nucleotide sequence