Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35445 and BPHYT_RS35450 are separated by 71 nucleotides BPHYT_RS35450 and BPHYT_RS35455 overlap by 4 nucleotides BPHYT_RS35455 and BPHYT_RS35460 overlap by 4 nucleotides BPHYT_RS35460 and BPHYT_RS35465 are separated by 1 nucleotides BPHYT_RS35465 and BPHYT_RS35470 are separated by 27 nucleotides
BPHYT_RS35445: BPHYT_RS35445 - 2-aminobenzoate-CoA ligase [EC: 6.2.1.32] <small>(from data)</small>, at 3,553,581 to 3,555,221
_RS35445
BPHYT_RS35450: BPHYT_RS35450 - endoribonuclease L-PSP, at 3,555,293 to 3,555,688
_RS35450
BPHYT_RS35455: BPHYT_RS35455 - 4-hydroxybenzoyl-CoA thioesterase, at 3,555,685 to 3,556,128
_RS35455
BPHYT_RS35460: BPHYT_RS35460 - acyl-CoA dehydrogenase, at 3,556,125 to 3,557,333
_RS35460
BPHYT_RS35465: BPHYT_RS35465 - enoyl-CoA hydratase, at 3,557,335 to 3,558,186
_RS35465
BPHYT_RS35470: BPHYT_RS35470 - MarR family transcriptional regulator, at 3,558,214 to 3,558,777
_RS35470
Position (kb) 3556
3557
3558 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3555.459 kb on + strand, within BPHYT_RS35450 at 3555.886 kb on - strand, within BPHYT_RS35455 at 3556.403 kb on - strand, within BPHYT_RS35460 at 3556.517 kb on + strand, within BPHYT_RS35460 at 3556.852 kb on + strand, within BPHYT_RS35460 at 3556.852 kb on + strand, within BPHYT_RS35460 at 3556.853 kb on - strand, within BPHYT_RS35460 at 3557.250 kb on - strand at 3557.737 kb on - strand, within BPHYT_RS35465 at 3557.737 kb on - strand, within BPHYT_RS35465 at 3557.996 kb on + strand, within BPHYT_RS35465 at 3557.997 kb on - strand, within BPHYT_RS35465
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 3,555,459 + BPHYT_RS35450 0.42 -2.2 3,555,886 - BPHYT_RS35455 0.45 +1.0 3,556,403 - BPHYT_RS35460 0.23 -0.6 3,556,517 + BPHYT_RS35460 0.32 -1.4 3,556,852 + BPHYT_RS35460 0.60 -1.0 3,556,852 + BPHYT_RS35460 0.60 -1.3 3,556,853 - BPHYT_RS35460 0.60 -0.5 3,557,250 - -3.2 3,557,737 - BPHYT_RS35465 0.47 -0.9 3,557,737 - BPHYT_RS35465 0.47 +0.2 3,557,996 + BPHYT_RS35465 0.78 -1.1 3,557,997 - BPHYT_RS35465 0.78 -0.9
Or see this region's nucleotide sequence