Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25540

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25530 and BPHYT_RS25535 overlap by 1 nucleotidesBPHYT_RS25535 and BPHYT_RS25540 are separated by 64 nucleotidesBPHYT_RS25540 and BPHYT_RS25545 are separated by 347 nucleotides BPHYT_RS25530: BPHYT_RS25530 - thioredoxin, at 1,301,432 to 1,301,821 _RS25530 BPHYT_RS25535: BPHYT_RS25535 - cytochrome C biogenesis protein, at 1,301,821 to 1,302,549 _RS25535 BPHYT_RS25540: BPHYT_RS25540 - phosphohydrolase, at 1,302,614 to 1,303,438 _RS25540 BPHYT_RS25545: BPHYT_RS25545 - hypothetical protein, at 1,303,786 to 1,305,726 _RS25545 Position (kb) 1302 1303 1304Strain fitness (log2 ratio) -1 0 1at 1301.849 kb on + strandat 1302.583 kb on - strandat 1302.768 kb on - strand, within BPHYT_RS25540at 1302.913 kb on - strand, within BPHYT_RS25540at 1302.998 kb on - strand, within BPHYT_RS25540at 1303.374 kb on + strandat 1303.418 kb on - strandat 1303.418 kb on - strandat 1303.859 kb on - strandat 1303.954 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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1,301,849 + +0.1
1,302,583 - +0.1
1,302,768 - BPHYT_RS25540 0.19 -1.7
1,302,913 - BPHYT_RS25540 0.36 -1.4
1,302,998 - BPHYT_RS25540 0.47 -1.4
1,303,374 + +0.9
1,303,418 - -0.1
1,303,418 - -0.8
1,303,859 - +0.4
1,303,954 - -0.3

Or see this region's nucleotide sequence