Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24895

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24890 and BPHYT_RS24895 are separated by 470 nucleotidesBPHYT_RS24895 and BPHYT_RS24900 overlap by 14 nucleotides BPHYT_RS24890: BPHYT_RS24890 - hydrolase, at 1,154,383 to 1,155,453 _RS24890 BPHYT_RS24895: BPHYT_RS24895 - multidrug ABC transporter substrate-binding protein, at 1,155,924 to 1,158,497 _RS24895 BPHYT_RS24900: BPHYT_RS24900 - ABC transporter ATP-binding protein, at 1,158,484 to 1,159,215 _RS24900 Position (kb) 1155 1156 1157 1158 1159Strain fitness (log2 ratio) -1 0 1 2at 1154.926 kb on - strand, within BPHYT_RS24890at 1154.926 kb on - strand, within BPHYT_RS24890at 1154.926 kb on - strand, within BPHYT_RS24890at 1154.927 kb on + strand, within BPHYT_RS24890at 1154.931 kb on - strand, within BPHYT_RS24890at 1154.933 kb on + strand, within BPHYT_RS24890at 1154.933 kb on + strand, within BPHYT_RS24890at 1154.933 kb on + strand, within BPHYT_RS24890at 1154.934 kb on - strand, within BPHYT_RS24890at 1154.934 kb on - strand, within BPHYT_RS24890at 1154.934 kb on - strand, within BPHYT_RS24890at 1154.934 kb on - strand, within BPHYT_RS24890at 1155.332 kb on + strand, within BPHYT_RS24890at 1155.332 kb on + strand, within BPHYT_RS24890at 1156.315 kb on + strand, within BPHYT_RS24895at 1156.316 kb on - strand, within BPHYT_RS24895at 1156.515 kb on + strand, within BPHYT_RS24895at 1156.544 kb on - strand, within BPHYT_RS24895at 1156.622 kb on - strand, within BPHYT_RS24895at 1156.628 kb on - strand, within BPHYT_RS24895at 1156.823 kb on + strand, within BPHYT_RS24895at 1157.370 kb on + strand, within BPHYT_RS24895at 1157.702 kb on + strand, within BPHYT_RS24895at 1157.703 kb on - strand, within BPHYT_RS24895at 1158.039 kb on + strand, within BPHYT_RS24895at 1158.040 kb on - strand, within BPHYT_RS24895at 1158.040 kb on - strand, within BPHYT_RS24895at 1158.381 kb on - strandat 1158.471 kb on + strandat 1159.157 kb on + strandat 1159.210 kb on + strandat 1159.210 kb on + strandat 1159.210 kb on + strandat 1159.210 kb on + strandat 1159.210 kb on + strandat 1159.322 kb on + strandat 1159.323 kb on - strandat 1159.323 kb on - strandat 1159.432 kb on + strandat 1159.437 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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1,154,926 - BPHYT_RS24890 0.51 +0.6
1,154,926 - BPHYT_RS24890 0.51 +1.2
1,154,926 - BPHYT_RS24890 0.51 +0.0
1,154,927 + BPHYT_RS24890 0.51 -0.6
1,154,931 - BPHYT_RS24890 0.51 -0.8
1,154,933 + BPHYT_RS24890 0.51 +1.1
1,154,933 + BPHYT_RS24890 0.51 +0.2
1,154,933 + BPHYT_RS24890 0.51 -0.2
1,154,934 - BPHYT_RS24890 0.51 -0.5
1,154,934 - BPHYT_RS24890 0.51 +1.4
1,154,934 - BPHYT_RS24890 0.51 +0.2
1,154,934 - BPHYT_RS24890 0.51 -0.6
1,155,332 + BPHYT_RS24890 0.89 +1.1
1,155,332 + BPHYT_RS24890 0.89 -0.3
1,156,315 + BPHYT_RS24895 0.15 +0.7
1,156,316 - BPHYT_RS24895 0.15 -0.4
1,156,515 + BPHYT_RS24895 0.23 +0.2
1,156,544 - BPHYT_RS24895 0.24 +0.4
1,156,622 - BPHYT_RS24895 0.27 +0.2
1,156,628 - BPHYT_RS24895 0.27 +0.2
1,156,823 + BPHYT_RS24895 0.35 +0.6
1,157,370 + BPHYT_RS24895 0.56 +1.0
1,157,702 + BPHYT_RS24895 0.69 -0.1
1,157,703 - BPHYT_RS24895 0.69 +1.1
1,158,039 + BPHYT_RS24895 0.82 +0.5
1,158,040 - BPHYT_RS24895 0.82 +0.0
1,158,040 - BPHYT_RS24895 0.82 -0.7
1,158,381 - -0.3
1,158,471 + +0.9
1,159,157 + -0.4
1,159,210 + +0.1
1,159,210 + -1.1
1,159,210 + -0.3
1,159,210 + +0.7
1,159,210 + +0.2
1,159,322 + -0.0
1,159,323 - +0.1
1,159,323 - -0.3
1,159,432 + +1.8
1,159,437 - -0.0

Or see this region's nucleotide sequence