Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24890 and BPHYT_RS24895 are separated by 470 nucleotides BPHYT_RS24895 and BPHYT_RS24900 overlap by 14 nucleotides
BPHYT_RS24890: BPHYT_RS24890 - hydrolase, at 1,154,383 to 1,155,453
_RS24890
BPHYT_RS24895: BPHYT_RS24895 - multidrug ABC transporter substrate-binding protein, at 1,155,924 to 1,158,497
_RS24895
BPHYT_RS24900: BPHYT_RS24900 - ABC transporter ATP-binding protein, at 1,158,484 to 1,159,215
_RS24900
Position (kb)
1155
1156
1157
1158
1159 Strain fitness (log2 ratio)
-1
0
1
2 at 1154.926 kb on - strand, within BPHYT_RS24890 at 1154.926 kb on - strand, within BPHYT_RS24890 at 1154.926 kb on - strand, within BPHYT_RS24890 at 1154.927 kb on + strand, within BPHYT_RS24890 at 1154.931 kb on - strand, within BPHYT_RS24890 at 1154.933 kb on + strand, within BPHYT_RS24890 at 1154.933 kb on + strand, within BPHYT_RS24890 at 1154.933 kb on + strand, within BPHYT_RS24890 at 1154.934 kb on - strand, within BPHYT_RS24890 at 1154.934 kb on - strand, within BPHYT_RS24890 at 1154.934 kb on - strand, within BPHYT_RS24890 at 1154.934 kb on - strand, within BPHYT_RS24890 at 1155.332 kb on + strand, within BPHYT_RS24890 at 1155.332 kb on + strand, within BPHYT_RS24890 at 1156.315 kb on + strand, within BPHYT_RS24895 at 1156.316 kb on - strand, within BPHYT_RS24895 at 1156.515 kb on + strand, within BPHYT_RS24895 at 1156.544 kb on - strand, within BPHYT_RS24895 at 1156.622 kb on - strand, within BPHYT_RS24895 at 1156.628 kb on - strand, within BPHYT_RS24895 at 1156.823 kb on + strand, within BPHYT_RS24895 at 1157.370 kb on + strand, within BPHYT_RS24895 at 1157.702 kb on + strand, within BPHYT_RS24895 at 1157.703 kb on - strand, within BPHYT_RS24895 at 1158.039 kb on + strand, within BPHYT_RS24895 at 1158.040 kb on - strand, within BPHYT_RS24895 at 1158.040 kb on - strand, within BPHYT_RS24895 at 1158.381 kb on - strand at 1158.471 kb on + strand at 1159.157 kb on + strand at 1159.210 kb on + strand at 1159.210 kb on + strand at 1159.210 kb on + strand at 1159.210 kb on + strand at 1159.210 kb on + strand at 1159.322 kb on + strand at 1159.323 kb on - strand at 1159.323 kb on - strand at 1159.432 kb on + strand at 1159.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 1,154,926 - BPHYT_RS24890 0.51 +0.6 1,154,926 - BPHYT_RS24890 0.51 +1.2 1,154,926 - BPHYT_RS24890 0.51 +0.0 1,154,927 + BPHYT_RS24890 0.51 -0.6 1,154,931 - BPHYT_RS24890 0.51 -0.8 1,154,933 + BPHYT_RS24890 0.51 +1.1 1,154,933 + BPHYT_RS24890 0.51 +0.2 1,154,933 + BPHYT_RS24890 0.51 -0.2 1,154,934 - BPHYT_RS24890 0.51 -0.5 1,154,934 - BPHYT_RS24890 0.51 +1.4 1,154,934 - BPHYT_RS24890 0.51 +0.2 1,154,934 - BPHYT_RS24890 0.51 -0.6 1,155,332 + BPHYT_RS24890 0.89 +1.1 1,155,332 + BPHYT_RS24890 0.89 -0.3 1,156,315 + BPHYT_RS24895 0.15 +0.7 1,156,316 - BPHYT_RS24895 0.15 -0.4 1,156,515 + BPHYT_RS24895 0.23 +0.2 1,156,544 - BPHYT_RS24895 0.24 +0.4 1,156,622 - BPHYT_RS24895 0.27 +0.2 1,156,628 - BPHYT_RS24895 0.27 +0.2 1,156,823 + BPHYT_RS24895 0.35 +0.6 1,157,370 + BPHYT_RS24895 0.56 +1.0 1,157,702 + BPHYT_RS24895 0.69 -0.1 1,157,703 - BPHYT_RS24895 0.69 +1.1 1,158,039 + BPHYT_RS24895 0.82 +0.5 1,158,040 - BPHYT_RS24895 0.82 +0.0 1,158,040 - BPHYT_RS24895 0.82 -0.7 1,158,381 - -0.3 1,158,471 + +0.9 1,159,157 + -0.4 1,159,210 + +0.1 1,159,210 + -1.1 1,159,210 + -0.3 1,159,210 + +0.7 1,159,210 + +0.2 1,159,322 + -0.0 1,159,323 - +0.1 1,159,323 - -0.3 1,159,432 + +1.8 1,159,437 - -0.0
Or see this region's nucleotide sequence