Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24685 and BPHYT_RS24690 are separated by 54 nucleotides BPHYT_RS24690 and BPHYT_RS24695 are separated by 477 nucleotides
BPHYT_RS24685: BPHYT_RS24685 - LysR family transcriptional regulator, at 1,106,955 to 1,107,848
_RS24685
BPHYT_RS24690: BPHYT_RS24690 - ionic transporter y4hA, at 1,107,903 to 1,108,985
_RS24690
BPHYT_RS24695: BPHYT_RS24695 - hypothetical protein, at 1,109,463 to 1,109,810
_RS24695
Position (kb)
1107
1108
1109 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1107.040 kb on + strand at 1107.093 kb on + strand, within BPHYT_RS24685 at 1107.094 kb on - strand, within BPHYT_RS24685 at 1107.384 kb on + strand, within BPHYT_RS24685 at 1107.385 kb on - strand, within BPHYT_RS24685 at 1107.509 kb on + strand, within BPHYT_RS24685 at 1107.656 kb on - strand, within BPHYT_RS24685 at 1108.224 kb on - strand, within BPHYT_RS24690 at 1108.409 kb on - strand, within BPHYT_RS24690 at 1108.437 kb on + strand, within BPHYT_RS24690 at 1108.450 kb on + strand, within BPHYT_RS24690 at 1108.450 kb on + strand, within BPHYT_RS24690 at 1108.525 kb on + strand, within BPHYT_RS24690 at 1108.525 kb on + strand, within BPHYT_RS24690 at 1108.525 kb on + strand, within BPHYT_RS24690 at 1108.526 kb on - strand, within BPHYT_RS24690 at 1108.526 kb on - strand, within BPHYT_RS24690 at 1109.095 kb on + strand at 1109.369 kb on + strand at 1109.369 kb on + strand at 1109.370 kb on - strand at 1109.372 kb on + strand at 1109.372 kb on + strand at 1109.744 kb on - strand at 1109.823 kb on + strand at 1109.823 kb on + strand at 1109.823 kb on + strand at 1109.824 kb on - strand at 1109.824 kb on - strand at 1109.836 kb on - strand at 1109.836 kb on - strand at 1109.865 kb on - strand at 1109.865 kb on - strand at 1109.877 kb on - strand at 1109.927 kb on - strand at 1109.936 kb on + strand at 1109.936 kb on + strand at 1109.936 kb on + strand at 1109.936 kb on + strand at 1109.937 kb on - strand at 1109.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 1,107,040 + -0.4 1,107,093 + BPHYT_RS24685 0.15 -1.3 1,107,094 - BPHYT_RS24685 0.16 +0.4 1,107,384 + BPHYT_RS24685 0.48 +0.7 1,107,385 - BPHYT_RS24685 0.48 +0.9 1,107,509 + BPHYT_RS24685 0.62 +0.3 1,107,656 - BPHYT_RS24685 0.78 -0.0 1,108,224 - BPHYT_RS24690 0.30 +1.0 1,108,409 - BPHYT_RS24690 0.47 +0.8 1,108,437 + BPHYT_RS24690 0.49 -0.7 1,108,450 + BPHYT_RS24690 0.51 +2.2 1,108,450 + BPHYT_RS24690 0.51 +0.7 1,108,525 + BPHYT_RS24690 0.57 +0.9 1,108,525 + BPHYT_RS24690 0.57 +0.4 1,108,525 + BPHYT_RS24690 0.57 +0.8 1,108,526 - BPHYT_RS24690 0.58 +0.7 1,108,526 - BPHYT_RS24690 0.58 +0.6 1,109,095 + -0.3 1,109,369 + +0.1 1,109,369 + +0.9 1,109,370 - -0.5 1,109,372 + +0.0 1,109,372 + -0.2 1,109,744 - +3.1 1,109,823 + -0.5 1,109,823 + -0.3 1,109,823 + +0.5 1,109,824 - -1.0 1,109,824 - -0.5 1,109,836 - -0.8 1,109,836 - -2.2 1,109,865 - -0.2 1,109,865 - -0.1 1,109,877 - -0.1 1,109,927 - -0.4 1,109,936 + -0.4 1,109,936 + +0.2 1,109,936 + -0.9 1,109,936 + +0.0 1,109,937 - +2.1 1,109,937 - -0.1
Or see this region's nucleotide sequence