Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24445 and BPHYT_RS24450 are separated by 259 nucleotides BPHYT_RS24450 and BPHYT_RS24455 are separated by 124 nucleotides
BPHYT_RS24445: BPHYT_RS24445 - type VI secretion protein VasK, at 1,050,281 to 1,054,147
_RS24445
BPHYT_RS24450: BPHYT_RS24450 - D-alanyl-D-alanine carboxypeptidase, at 1,054,407 to 1,055,252
_RS24450
BPHYT_RS24455: BPHYT_RS24455 - hypothetical protein, at 1,055,377 to 1,056,312
_RS24455
Position (kb)
1054
1055
1056 Strain fitness (log2 ratio)
-2
-1
0
1 at 1053.592 kb on - strand, within BPHYT_RS24445 at 1053.646 kb on + strand, within BPHYT_RS24445 at 1053.647 kb on - strand, within BPHYT_RS24445 at 1054.055 kb on - strand at 1054.070 kb on + strand at 1054.071 kb on - strand at 1054.147 kb on + strand at 1054.225 kb on + strand at 1054.382 kb on - strand at 1054.425 kb on + strand at 1054.425 kb on + strand at 1054.521 kb on + strand, within BPHYT_RS24450 at 1054.522 kb on - strand, within BPHYT_RS24450 at 1054.529 kb on - strand, within BPHYT_RS24450 at 1054.609 kb on + strand, within BPHYT_RS24450 at 1054.619 kb on - strand, within BPHYT_RS24450 at 1054.744 kb on + strand, within BPHYT_RS24450 at 1054.849 kb on - strand, within BPHYT_RS24450 at 1054.998 kb on - strand, within BPHYT_RS24450 at 1055.114 kb on + strand, within BPHYT_RS24450 at 1055.133 kb on - strand, within BPHYT_RS24450 at 1055.163 kb on + strand, within BPHYT_RS24450 at 1055.166 kb on + strand, within BPHYT_RS24450 at 1055.166 kb on + strand, within BPHYT_RS24450 at 1055.178 kb on + strand at 1055.190 kb on + strand at 1055.239 kb on - strand at 1055.242 kb on - strand at 1055.281 kb on + strand at 1055.289 kb on - strand at 1055.335 kb on + strand at 1056.074 kb on + strand, within BPHYT_RS24455 at 1056.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 1,053,592 - BPHYT_RS24445 0.86 -0.4 1,053,646 + BPHYT_RS24445 0.87 -0.7 1,053,647 - BPHYT_RS24445 0.87 -1.1 1,054,055 - +0.1 1,054,070 + +0.5 1,054,071 - +0.1 1,054,147 + -0.0 1,054,225 + -0.7 1,054,382 - +0.1 1,054,425 + -0.2 1,054,425 + -1.7 1,054,521 + BPHYT_RS24450 0.13 -0.8 1,054,522 - BPHYT_RS24450 0.14 -1.3 1,054,529 - BPHYT_RS24450 0.14 -0.6 1,054,609 + BPHYT_RS24450 0.24 -0.9 1,054,619 - BPHYT_RS24450 0.25 -1.4 1,054,744 + BPHYT_RS24450 0.40 -2.0 1,054,849 - BPHYT_RS24450 0.52 +0.1 1,054,998 - BPHYT_RS24450 0.70 +0.2 1,055,114 + BPHYT_RS24450 0.84 -0.6 1,055,133 - BPHYT_RS24450 0.86 -0.6 1,055,163 + BPHYT_RS24450 0.89 +1.2 1,055,166 + BPHYT_RS24450 0.90 +0.2 1,055,166 + BPHYT_RS24450 0.90 -0.0 1,055,178 + +0.1 1,055,190 + -0.3 1,055,239 - +0.3 1,055,242 - +0.1 1,055,281 + -0.6 1,055,289 - -1.8 1,055,335 + -0.8 1,056,074 + BPHYT_RS24455 0.74 -0.1 1,056,246 - +0.0
Or see this region's nucleotide sequence