Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23445

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23440 and BPHYT_RS23445 are separated by 108 nucleotidesBPHYT_RS23445 and BPHYT_RS23450 are separated by 216 nucleotides BPHYT_RS23440: BPHYT_RS23440 - alcohol dehydrogenase, at 826,689 to 827,735 _RS23440 BPHYT_RS23445: BPHYT_RS23445 - hexuronate transporter, at 827,844 to 829,136 _RS23445 BPHYT_RS23450: BPHYT_RS23450 - GntR family transcriptional regulator, at 829,353 to 830,066 _RS23450 Position (kb) 827 828 829 830Strain fitness (log2 ratio) -1 0 1 2at 827.046 kb on - strand, within BPHYT_RS23440at 827.586 kb on + strand, within BPHYT_RS23440at 827.587 kb on - strand, within BPHYT_RS23440at 827.587 kb on - strand, within BPHYT_RS23440at 827.905 kb on - strandat 828.564 kb on + strand, within BPHYT_RS23445at 828.564 kb on + strand, within BPHYT_RS23445at 829.135 kb on - strandat 829.157 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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827,046 - BPHYT_RS23440 0.34 -0.3
827,586 + BPHYT_RS23440 0.86 -0.5
827,587 - BPHYT_RS23440 0.86 +1.9
827,587 - BPHYT_RS23440 0.86 -0.9
827,905 - -0.5
828,564 + BPHYT_RS23445 0.56 -0.8
828,564 + BPHYT_RS23445 0.56 -0.3
829,135 - -0.5
829,157 + -1.6

Or see this region's nucleotide sequence