Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21310 and BPHYT_RS21315 are separated by 628 nucleotides BPHYT_RS21315 and BPHYT_RS21320 are separated by 216 nucleotides
BPHYT_RS21310: BPHYT_RS21310 - porin, at 339,621 to 340,772
_RS21310
BPHYT_RS21315: BPHYT_RS21315 - hypothetical protein, at 341,401 to 341,937
_RS21315
BPHYT_RS21320: BPHYT_RS21320 - alkaline phosphatase, at 342,154 to 344,106
_RS21320
Position (kb) 341
342 Strain fitness (log2 ratio) -2
-1
0
1 at 340.409 kb on - strand, within BPHYT_RS21310 at 340.427 kb on - strand, within BPHYT_RS21310 at 340.454 kb on - strand, within BPHYT_RS21310 at 340.454 kb on - strand, within BPHYT_RS21310 at 340.603 kb on + strand, within BPHYT_RS21310 at 340.603 kb on + strand, within BPHYT_RS21310 at 340.604 kb on - strand, within BPHYT_RS21310 at 340.604 kb on - strand, within BPHYT_RS21310 at 340.693 kb on + strand at 340.693 kb on + strand at 340.694 kb on - strand at 340.694 kb on - strand at 340.781 kb on - strand at 340.812 kb on - strand at 340.978 kb on + strand at 341.052 kb on - strand at 341.127 kb on + strand at 341.245 kb on + strand at 341.245 kb on + strand at 341.245 kb on + strand at 341.727 kb on + strand, within BPHYT_RS21315 at 341.809 kb on + strand, within BPHYT_RS21315 at 341.833 kb on - strand, within BPHYT_RS21315 at 341.833 kb on - strand, within BPHYT_RS21315 at 341.872 kb on - strand, within BPHYT_RS21315 at 341.872 kb on - strand, within BPHYT_RS21315 at 342.010 kb on + strand at 342.010 kb on + strand at 342.014 kb on - strand at 342.014 kb on - strand at 342.026 kb on + strand at 342.028 kb on + strand at 342.029 kb on - strand at 342.048 kb on - strand at 342.048 kb on - strand at 342.439 kb on + strand, within BPHYT_RS21320 at 342.440 kb on - strand, within BPHYT_RS21320 at 342.571 kb on + strand, within BPHYT_RS21320 at 342.703 kb on + strand, within BPHYT_RS21320 at 342.703 kb on + strand, within BPHYT_RS21320 at 342.704 kb on - strand, within BPHYT_RS21320 at 342.781 kb on + strand, within BPHYT_RS21320 at 342.796 kb on - strand, within BPHYT_RS21320
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 340,409 - BPHYT_RS21310 0.68 -0.1 340,427 - BPHYT_RS21310 0.70 -0.4 340,454 - BPHYT_RS21310 0.72 -1.2 340,454 - BPHYT_RS21310 0.72 -0.0 340,603 + BPHYT_RS21310 0.85 +0.9 340,603 + BPHYT_RS21310 0.85 -1.1 340,604 - BPHYT_RS21310 0.85 +0.5 340,604 - BPHYT_RS21310 0.85 -0.1 340,693 + +1.1 340,693 + -0.7 340,694 - -0.5 340,694 - -0.1 340,781 - -0.6 340,812 - -1.1 340,978 + +0.0 341,052 - +0.1 341,127 + +0.5 341,245 + +0.2 341,245 + -0.0 341,245 + -0.3 341,727 + BPHYT_RS21315 0.61 -2.3 341,809 + BPHYT_RS21315 0.76 +0.4 341,833 - BPHYT_RS21315 0.80 -0.3 341,833 - BPHYT_RS21315 0.80 -0.0 341,872 - BPHYT_RS21315 0.88 -0.3 341,872 - BPHYT_RS21315 0.88 +0.5 342,010 + -0.1 342,010 + -0.6 342,014 - +0.7 342,014 - +0.0 342,026 + -0.2 342,028 + +0.8 342,029 - +0.2 342,048 - +0.3 342,048 - -0.4 342,439 + BPHYT_RS21320 0.15 +0.3 342,440 - BPHYT_RS21320 0.15 +1.1 342,571 + BPHYT_RS21320 0.21 -0.3 342,703 + BPHYT_RS21320 0.28 +1.7 342,703 + BPHYT_RS21320 0.28 -2.1 342,704 - BPHYT_RS21320 0.28 +0.1 342,781 + BPHYT_RS21320 0.32 +0.2 342,796 - BPHYT_RS21320 0.33 +1.1
Or see this region's nucleotide sequence