Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21295 and BPHYT_RS21300 are separated by 45 nucleotides BPHYT_RS21300 and BPHYT_RS21305 are separated by 139 nucleotides BPHYT_RS21305 and BPHYT_RS21310 are separated by 165 nucleotides
BPHYT_RS21295: BPHYT_RS21295 - AraC family transcriptional regulator, at 337,430 to 338,251
_RS21295
BPHYT_RS21300: BPHYT_RS21300 - membrane protein, at 338,297 to 338,812
_RS21300
BPHYT_RS21305: BPHYT_RS21305 - hypothetical protein, at 338,952 to 339,455
_RS21305
BPHYT_RS21310: BPHYT_RS21310 - porin, at 339,621 to 340,772
_RS21310
Position (kb) 338
339
340 Strain fitness (log2 ratio) -1
0
1
2
3 at 338.084 kb on + strand, within BPHYT_RS21295 at 338.084 kb on + strand, within BPHYT_RS21295 at 338.085 kb on - strand, within BPHYT_RS21295 at 338.232 kb on - strand at 338.420 kb on - strand, within BPHYT_RS21300 at 338.466 kb on - strand, within BPHYT_RS21300 at 338.536 kb on - strand, within BPHYT_RS21300 at 338.855 kb on + strand at 339.074 kb on - strand, within BPHYT_RS21305 at 339.131 kb on + strand, within BPHYT_RS21305 at 339.131 kb on + strand, within BPHYT_RS21305 at 339.131 kb on + strand, within BPHYT_RS21305 at 339.172 kb on + strand, within BPHYT_RS21305 at 339.173 kb on - strand, within BPHYT_RS21305 at 339.199 kb on + strand, within BPHYT_RS21305 at 339.199 kb on + strand, within BPHYT_RS21305 at 339.200 kb on - strand, within BPHYT_RS21305 at 339.200 kb on - strand, within BPHYT_RS21305 at 339.200 kb on - strand, within BPHYT_RS21305 at 339.200 kb on - strand, within BPHYT_RS21305 at 339.200 kb on - strand, within BPHYT_RS21305 at 339.200 kb on - strand, within BPHYT_RS21305 at 339.201 kb on + strand, within BPHYT_RS21305 at 339.371 kb on - strand, within BPHYT_RS21305 at 339.467 kb on - strand at 339.525 kb on + strand at 339.606 kb on - strand at 339.623 kb on - strand at 339.742 kb on + strand, within BPHYT_RS21310 at 339.742 kb on + strand, within BPHYT_RS21310 at 339.743 kb on - strand, within BPHYT_RS21310 at 339.757 kb on + strand, within BPHYT_RS21310 at 339.758 kb on - strand, within BPHYT_RS21310 at 339.758 kb on - strand, within BPHYT_RS21310 at 339.758 kb on - strand, within BPHYT_RS21310 at 339.758 kb on - strand, within BPHYT_RS21310 at 339.784 kb on + strand, within BPHYT_RS21310 at 339.865 kb on + strand, within BPHYT_RS21310 at 339.865 kb on + strand, within BPHYT_RS21310 at 339.865 kb on + strand, within BPHYT_RS21310 at 339.919 kb on + strand, within BPHYT_RS21310 at 339.920 kb on - strand, within BPHYT_RS21310 at 340.010 kb on + strand, within BPHYT_RS21310 at 340.010 kb on + strand, within BPHYT_RS21310 at 340.010 kb on + strand, within BPHYT_RS21310 at 340.011 kb on - strand, within BPHYT_RS21310 at 340.039 kb on + strand, within BPHYT_RS21310 at 340.040 kb on - strand, within BPHYT_RS21310 at 340.045 kb on + strand, within BPHYT_RS21310 at 340.046 kb on - strand, within BPHYT_RS21310 at 340.046 kb on - strand, within BPHYT_RS21310 at 340.046 kb on - strand, within BPHYT_RS21310 at 340.063 kb on + strand, within BPHYT_RS21310 at 340.192 kb on + strand, within BPHYT_RS21310 at 340.255 kb on + strand, within BPHYT_RS21310 at 340.255 kb on + strand, within BPHYT_RS21310 at 340.282 kb on + strand, within BPHYT_RS21310 at 340.283 kb on - strand, within BPHYT_RS21310 at 340.297 kb on + strand, within BPHYT_RS21310 at 340.298 kb on - strand, within BPHYT_RS21310 at 340.298 kb on - strand, within BPHYT_RS21310 at 340.298 kb on - strand, within BPHYT_RS21310 at 340.409 kb on - strand, within BPHYT_RS21310 at 340.427 kb on - strand, within BPHYT_RS21310 at 340.454 kb on - strand, within BPHYT_RS21310 at 340.454 kb on - strand, within BPHYT_RS21310
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 338,084 + BPHYT_RS21295 0.80 -0.4 338,084 + BPHYT_RS21295 0.80 +0.1 338,085 - BPHYT_RS21295 0.80 -0.3 338,232 - -0.7 338,420 - BPHYT_RS21300 0.24 +0.3 338,466 - BPHYT_RS21300 0.33 +2.2 338,536 - BPHYT_RS21300 0.46 +0.1 338,855 + -0.3 339,074 - BPHYT_RS21305 0.24 +0.6 339,131 + BPHYT_RS21305 0.36 +0.2 339,131 + BPHYT_RS21305 0.36 -0.4 339,131 + BPHYT_RS21305 0.36 -0.7 339,172 + BPHYT_RS21305 0.44 +0.4 339,173 - BPHYT_RS21305 0.44 -1.1 339,199 + BPHYT_RS21305 0.49 -1.5 339,199 + BPHYT_RS21305 0.49 +0.5 339,200 - BPHYT_RS21305 0.49 -0.7 339,200 - BPHYT_RS21305 0.49 +1.0 339,200 - BPHYT_RS21305 0.49 +0.2 339,200 - BPHYT_RS21305 0.49 +1.0 339,200 - BPHYT_RS21305 0.49 -1.5 339,200 - BPHYT_RS21305 0.49 +0.4 339,201 + BPHYT_RS21305 0.49 -0.1 339,371 - BPHYT_RS21305 0.83 +0.6 339,467 - +0.3 339,525 + +0.6 339,606 - +0.4 339,623 - -0.7 339,742 + BPHYT_RS21310 0.11 +0.6 339,742 + BPHYT_RS21310 0.11 +3.1 339,743 - BPHYT_RS21310 0.11 -0.6 339,757 + BPHYT_RS21310 0.12 +1.6 339,758 - BPHYT_RS21310 0.12 -1.7 339,758 - BPHYT_RS21310 0.12 -0.3 339,758 - BPHYT_RS21310 0.12 -0.1 339,758 - BPHYT_RS21310 0.12 -0.1 339,784 + BPHYT_RS21310 0.14 +0.1 339,865 + BPHYT_RS21310 0.21 -0.3 339,865 + BPHYT_RS21310 0.21 -0.2 339,865 + BPHYT_RS21310 0.21 -0.9 339,919 + BPHYT_RS21310 0.26 -0.5 339,920 - BPHYT_RS21310 0.26 +0.0 340,010 + BPHYT_RS21310 0.34 +0.0 340,010 + BPHYT_RS21310 0.34 -0.4 340,010 + BPHYT_RS21310 0.34 +0.2 340,011 - BPHYT_RS21310 0.34 -0.0 340,039 + BPHYT_RS21310 0.36 -0.9 340,040 - BPHYT_RS21310 0.36 +0.3 340,045 + BPHYT_RS21310 0.37 -1.2 340,046 - BPHYT_RS21310 0.37 +0.5 340,046 - BPHYT_RS21310 0.37 -1.0 340,046 - BPHYT_RS21310 0.37 -0.2 340,063 + BPHYT_RS21310 0.38 +0.2 340,192 + BPHYT_RS21310 0.50 -0.3 340,255 + BPHYT_RS21310 0.55 +1.7 340,255 + BPHYT_RS21310 0.55 -0.1 340,282 + BPHYT_RS21310 0.57 -0.1 340,283 - BPHYT_RS21310 0.57 -0.5 340,297 + BPHYT_RS21310 0.59 +0.6 340,298 - BPHYT_RS21310 0.59 -0.3 340,298 - BPHYT_RS21310 0.59 +0.3 340,298 - BPHYT_RS21310 0.59 -0.1 340,409 - BPHYT_RS21310 0.68 -0.1 340,427 - BPHYT_RS21310 0.70 -0.4 340,454 - BPHYT_RS21310 0.72 -1.2 340,454 - BPHYT_RS21310 0.72 -0.0
Or see this region's nucleotide sequence