Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20845 and BPHYT_RS20850 are separated by 351 nucleotides BPHYT_RS20850 and BPHYT_RS20855 are separated by 126 nucleotides
BPHYT_RS20845: BPHYT_RS20845 - peroxidase, at 239,273 to 239,929
_RS20845
BPHYT_RS20850: BPHYT_RS20850 - multidrug DMT transporter permease, at 240,281 to 241,123
_RS20850
BPHYT_RS20855: BPHYT_RS20855 - transcriptional regulator, at 241,250 to 242,158
_RS20855
Position (kb) 240
241
242 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 239.399 kb on + strand, within BPHYT_RS20845 at 239.457 kb on - strand, within BPHYT_RS20845 at 239.934 kb on - strand at 239.942 kb on + strand at 240.069 kb on - strand at 240.069 kb on - strand at 240.069 kb on - strand at 240.155 kb on + strand at 240.156 kb on - strand at 240.190 kb on - strand at 240.460 kb on - strand, within BPHYT_RS20850 at 240.606 kb on + strand, within BPHYT_RS20850 at 240.661 kb on - strand, within BPHYT_RS20850 at 240.661 kb on - strand, within BPHYT_RS20850 at 241.184 kb on + strand at 241.184 kb on + strand at 241.185 kb on - strand at 241.188 kb on + strand at 241.196 kb on - strand at 241.216 kb on - strand at 241.216 kb on - strand at 241.339 kb on - strand at 241.673 kb on + strand, within BPHYT_RS20855 at 241.674 kb on - strand, within BPHYT_RS20855 at 241.709 kb on + strand, within BPHYT_RS20855 at 241.709 kb on + strand, within BPHYT_RS20855 at 241.709 kb on + strand, within BPHYT_RS20855 at 241.710 kb on - strand, within BPHYT_RS20855 at 241.710 kb on - strand, within BPHYT_RS20855 at 241.710 kb on - strand, within BPHYT_RS20855 at 241.715 kb on + strand, within BPHYT_RS20855 at 241.829 kb on - strand, within BPHYT_RS20855 at 241.953 kb on + strand, within BPHYT_RS20855 at 241.954 kb on - strand, within BPHYT_RS20855 at 242.060 kb on + strand, within BPHYT_RS20855 at 242.061 kb on - strand, within BPHYT_RS20855
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 239,399 + BPHYT_RS20845 0.19 -2.1 239,457 - BPHYT_RS20845 0.28 -0.2 239,934 - +2.8 239,942 + +1.3 240,069 - -1.3 240,069 - +0.6 240,069 - -0.6 240,155 + +0.5 240,156 - -0.7 240,190 - -0.0 240,460 - BPHYT_RS20850 0.21 -0.2 240,606 + BPHYT_RS20850 0.39 -0.9 240,661 - BPHYT_RS20850 0.45 -0.0 240,661 - BPHYT_RS20850 0.45 +0.4 241,184 + -0.4 241,184 + -1.2 241,185 - -2.1 241,188 + +1.1 241,196 - -0.4 241,216 - +0.4 241,216 - -0.1 241,339 - -0.5 241,673 + BPHYT_RS20855 0.47 -0.4 241,674 - BPHYT_RS20855 0.47 -2.0 241,709 + BPHYT_RS20855 0.50 -0.1 241,709 + BPHYT_RS20855 0.50 +0.1 241,709 + BPHYT_RS20855 0.50 -0.1 241,710 - BPHYT_RS20855 0.51 -1.0 241,710 - BPHYT_RS20855 0.51 -0.7 241,710 - BPHYT_RS20855 0.51 +0.3 241,715 + BPHYT_RS20855 0.51 -0.3 241,829 - BPHYT_RS20855 0.64 -1.1 241,953 + BPHYT_RS20855 0.77 -1.1 241,954 - BPHYT_RS20855 0.77 -0.7 242,060 + BPHYT_RS20855 0.89 -0.1 242,061 - BPHYT_RS20855 0.89 -0.3
Or see this region's nucleotide sequence