Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20845

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20840 and BPHYT_RS20845 are separated by 148 nucleotidesBPHYT_RS20845 and BPHYT_RS20850 are separated by 351 nucleotides BPHYT_RS20840: BPHYT_RS20840 - beta-lactamase, at 238,054 to 239,124 _RS20840 BPHYT_RS20845: BPHYT_RS20845 - peroxidase, at 239,273 to 239,929 _RS20845 BPHYT_RS20850: BPHYT_RS20850 - multidrug DMT transporter permease, at 240,281 to 241,123 _RS20850 Position (kb) 239 240Strain fitness (log2 ratio) -2 -1 0 1 2 3at 238.469 kb on - strand, within BPHYT_RS20840at 238.906 kb on + strand, within BPHYT_RS20840at 238.969 kb on - strand, within BPHYT_RS20840at 239.030 kb on - strandat 239.085 kb on - strandat 239.197 kb on + strandat 239.399 kb on + strand, within BPHYT_RS20845at 239.457 kb on - strand, within BPHYT_RS20845at 239.934 kb on - strandat 239.942 kb on + strandat 240.069 kb on - strandat 240.069 kb on - strandat 240.069 kb on - strandat 240.155 kb on + strandat 240.156 kb on - strandat 240.190 kb on - strandat 240.460 kb on - strand, within BPHYT_RS20850at 240.606 kb on + strand, within BPHYT_RS20850at 240.661 kb on - strand, within BPHYT_RS20850at 240.661 kb on - strand, within BPHYT_RS20850

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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238,469 - BPHYT_RS20840 0.39 +0.1
238,906 + BPHYT_RS20840 0.80 +1.0
238,969 - BPHYT_RS20840 0.85 -1.2
239,030 - -1.1
239,085 - -0.0
239,197 + +0.0
239,399 + BPHYT_RS20845 0.19 -2.1
239,457 - BPHYT_RS20845 0.28 -0.2
239,934 - +2.8
239,942 + +1.3
240,069 - -1.3
240,069 - +0.6
240,069 - -0.6
240,155 + +0.5
240,156 - -0.7
240,190 - -0.0
240,460 - BPHYT_RS20850 0.21 -0.2
240,606 + BPHYT_RS20850 0.39 -0.9
240,661 - BPHYT_RS20850 0.45 -0.0
240,661 - BPHYT_RS20850 0.45 +0.4

Or see this region's nucleotide sequence