Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11565

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11560 and BPHYT_RS11565 are separated by 26 nucleotidesBPHYT_RS11565 and BPHYT_RS11570 are separated by 333 nucleotides BPHYT_RS11560: BPHYT_RS11560 - Asp/Glu racemase, at 2,627,605 to 2,628,471 _RS11560 BPHYT_RS11565: BPHYT_RS11565 - nitrate reductase, at 2,628,498 to 2,629,979 _RS11565 BPHYT_RS11570: BPHYT_RS11570 - GntR family transcriptional regulator, at 2,630,313 to 2,631,053 _RS11570 Position (kb) 2628 2629 2630Strain fitness (log2 ratio) -2 -1 0 1 2at 2627.583 kb on - strandat 2627.597 kb on - strandat 2627.995 kb on - strand, within BPHYT_RS11560at 2628.471 kb on + strandat 2628.518 kb on - strandat 2629.546 kb on + strand, within BPHYT_RS11565at 2629.776 kb on + strand, within BPHYT_RS11565at 2629.934 kb on - strandat 2630.061 kb on + strandat 2630.122 kb on + strandat 2630.122 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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2,627,583 - +1.5
2,627,597 - +0.3
2,627,995 - BPHYT_RS11560 0.45 +0.7
2,628,471 + +1.7
2,628,518 - +0.2
2,629,546 + BPHYT_RS11565 0.71 -2.0
2,629,776 + BPHYT_RS11565 0.86 -0.8
2,629,934 - -1.2
2,630,061 + -0.4
2,630,122 + +0.1
2,630,122 + +1.8

Or see this region's nucleotide sequence