Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09025

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09020 and BPHYT_RS09025 are separated by 294 nucleotidesBPHYT_RS09025 and BPHYT_RS09030 are separated by 235 nucleotides BPHYT_RS09020: BPHYT_RS09020 - UDP phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase, at 2,040,900 to 2,042,657 _RS09020 BPHYT_RS09025: BPHYT_RS09025 - 50S ribosomal protein L31 type B, at 2,042,952 to 2,043,218 _RS09025 BPHYT_RS09030: BPHYT_RS09030 - membrane protein, at 2,043,454 to 2,044,290 _RS09030 Position (kb) 2042 2043 2044Strain fitness (log2 ratio) -1 0 1at 2043.391 kb on - strandat 2043.477 kb on + strandat 2043.477 kb on + strandat 2043.507 kb on - strandat 2043.524 kb on + strandat 2043.569 kb on - strand, within BPHYT_RS09030at 2043.698 kb on + strand, within BPHYT_RS09030at 2043.698 kb on + strand, within BPHYT_RS09030at 2043.699 kb on - strand, within BPHYT_RS09030at 2043.833 kb on + strand, within BPHYT_RS09030at 2043.834 kb on - strand, within BPHYT_RS09030at 2043.834 kb on - strand, within BPHYT_RS09030at 2043.834 kb on - strand, within BPHYT_RS09030at 2043.983 kb on + strand, within BPHYT_RS09030

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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2,043,391 - -0.9
2,043,477 + -0.1
2,043,477 + -0.5
2,043,507 - +0.9
2,043,524 + +0.9
2,043,569 - BPHYT_RS09030 0.14 +0.5
2,043,698 + BPHYT_RS09030 0.29 -0.3
2,043,698 + BPHYT_RS09030 0.29 -0.5
2,043,699 - BPHYT_RS09030 0.29 +0.7
2,043,833 + BPHYT_RS09030 0.45 +0.3
2,043,834 - BPHYT_RS09030 0.45 +1.6
2,043,834 - BPHYT_RS09030 0.45 +1.0
2,043,834 - BPHYT_RS09030 0.45 -1.1
2,043,983 + BPHYT_RS09030 0.63 -1.1

Or see this region's nucleotide sequence