Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06815

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06810 and BPHYT_RS06815 are separated by 495 nucleotidesBPHYT_RS06815 and BPHYT_RS06820 are separated by 17 nucleotidesBPHYT_RS06820 and BPHYT_RS06825 are separated by 68 nucleotidesBPHYT_RS06825 and BPHYT_RS06830 are separated by 314 nucleotides BPHYT_RS06810: BPHYT_RS06810 - membrane protein, at 1,539,955 to 1,541,073 _RS06810 BPHYT_RS06815: BPHYT_RS06815 - 16S ribosomal RNA, at 1,541,569 to 1,543,163 _RS06815 BPHYT_RS06820: BPHYT_RS06820 - tRNA-Ile, at 1,543,181 to 1,543,257 _RS06820 BPHYT_RS06825: BPHYT_RS06825 - tRNA-Ala, at 1,543,326 to 1,543,401 _RS06825 BPHYT_RS06830: BPHYT_RS06830 - 23S ribosomal RNA, at 1,543,716 to 1,546,618 _RS06830 Position (kb) 1541 1542 1543 1544Strain fitness (log2 ratio) -2 -1 0 1 2at 1540.573 kb on - strand, within BPHYT_RS06810at 1540.757 kb on - strand, within BPHYT_RS06810at 1540.860 kb on + strand, within BPHYT_RS06810at 1540.861 kb on - strand, within BPHYT_RS06810at 1540.889 kb on + strand, within BPHYT_RS06810at 1540.889 kb on + strand, within BPHYT_RS06810at 1540.889 kb on + strand, within BPHYT_RS06810at 1540.889 kb on + strand, within BPHYT_RS06810at 1540.890 kb on - strand, within BPHYT_RS06810at 1540.890 kb on - strand, within BPHYT_RS06810at 1540.893 kb on + strand, within BPHYT_RS06810at 1540.893 kb on + strand, within BPHYT_RS06810at 1540.963 kb on + strandat 1540.963 kb on + strandat 1540.964 kb on - strandat 1540.964 kb on - strandat 1540.964 kb on - strandat 1540.965 kb on + strandat 1540.965 kb on + strandat 1540.965 kb on + strandat 1540.965 kb on + strandat 1540.965 kb on + strandat 1540.965 kb on + strandat 1540.966 kb on - strandat 1540.966 kb on - strandat 1540.966 kb on - strandat 1541.075 kb on + strandat 1541.076 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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1,540,573 - BPHYT_RS06810 0.55 +2.1
1,540,757 - BPHYT_RS06810 0.72 -0.4
1,540,860 + BPHYT_RS06810 0.81 +0.7
1,540,861 - BPHYT_RS06810 0.81 -1.3
1,540,889 + BPHYT_RS06810 0.83 -1.6
1,540,889 + BPHYT_RS06810 0.83 +0.1
1,540,889 + BPHYT_RS06810 0.83 -0.9
1,540,889 + BPHYT_RS06810 0.83 +1.9
1,540,890 - BPHYT_RS06810 0.84 -0.7
1,540,890 - BPHYT_RS06810 0.84 -2.7
1,540,893 + BPHYT_RS06810 0.84 +0.2
1,540,893 + BPHYT_RS06810 0.84 -1.9
1,540,963 + +0.6
1,540,963 + +0.0
1,540,964 - -0.5
1,540,964 - +0.1
1,540,964 - +0.4
1,540,965 + -0.4
1,540,965 + +2.1
1,540,965 + +0.9
1,540,965 + -1.1
1,540,965 + -0.4
1,540,965 + +0.4
1,540,966 - -0.2
1,540,966 - -2.1
1,540,966 - -0.9
1,541,075 + -0.3
1,541,076 - -0.7

Or see this region's nucleotide sequence