Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01460 and BPHYT_RS01465 are separated by 114 nucleotides BPHYT_RS01465 and BPHYT_RS01470 are separated by 103 nucleotides BPHYT_RS01470 and BPHYT_RS01475 are separated by 300 nucleotides
BPHYT_RS01460: BPHYT_RS01460 - methionyl-tRNA formyltransferase, at 333,741 to 334,727
_RS01460
BPHYT_RS01465: BPHYT_RS01465 - lysine transporter LysE, at 334,842 to 335,486
_RS01465
BPHYT_RS01470: BPHYT_RS01470 - protease HtpX homolog, at 335,590 to 336,447
_RS01470
BPHYT_RS01475: BPHYT_RS01475 - 16S rRNA methyltransferase, at 336,748 to 338,187
_RS01475
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1
2 at 334.725 kb on + strand at 334.725 kb on + strand at 334.726 kb on - strand at 334.802 kb on + strand at 334.813 kb on - strand at 334.920 kb on + strand, within BPHYT_RS01465 at 334.920 kb on + strand, within BPHYT_RS01465 at 334.920 kb on + strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.922 kb on + strand, within BPHYT_RS01465 at 334.922 kb on + strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 335.060 kb on + strand, within BPHYT_RS01465 at 335.060 kb on + strand, within BPHYT_RS01465 at 335.084 kb on + strand, within BPHYT_RS01465 at 335.085 kb on - strand, within BPHYT_RS01465 at 335.089 kb on - strand, within BPHYT_RS01465 at 335.089 kb on - strand, within BPHYT_RS01465 at 335.089 kb on - strand, within BPHYT_RS01465 at 335.098 kb on - strand, within BPHYT_RS01465 at 335.098 kb on - strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.237 kb on - strand, within BPHYT_RS01465 at 335.237 kb on - strand, within BPHYT_RS01465 at 335.237 kb on - strand, within BPHYT_RS01465 at 335.513 kb on + strand at 335.514 kb on - strand at 335.520 kb on + strand at 335.520 kb on + strand at 335.520 kb on + strand at 335.525 kb on + strand at 335.526 kb on - strand at 336.604 kb on + strand at 336.605 kb on - strand at 336.694 kb on - strand at 336.694 kb on - strand at 336.739 kb on - strand at 336.739 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 334,725 + +0.2 334,725 + -0.3 334,726 - -0.9 334,802 + +0.4 334,813 - +0.6 334,920 + BPHYT_RS01465 0.12 +0.5 334,920 + BPHYT_RS01465 0.12 -0.0 334,920 + BPHYT_RS01465 0.12 +0.4 334,921 - BPHYT_RS01465 0.12 -0.5 334,921 - BPHYT_RS01465 0.12 +0.1 334,921 - BPHYT_RS01465 0.12 -2.1 334,921 - BPHYT_RS01465 0.12 +0.7 334,921 - BPHYT_RS01465 0.12 -0.1 334,921 - BPHYT_RS01465 0.12 -1.4 334,922 + BPHYT_RS01465 0.12 -0.3 334,922 + BPHYT_RS01465 0.12 +0.8 334,923 - BPHYT_RS01465 0.13 -0.8 334,923 - BPHYT_RS01465 0.13 -1.0 334,923 - BPHYT_RS01465 0.13 +0.6 334,923 - BPHYT_RS01465 0.13 +0.2 334,923 - BPHYT_RS01465 0.13 -0.4 334,923 - BPHYT_RS01465 0.13 -0.2 335,060 + BPHYT_RS01465 0.34 -0.7 335,060 + BPHYT_RS01465 0.34 -0.1 335,084 + BPHYT_RS01465 0.38 +1.1 335,085 - BPHYT_RS01465 0.38 +1.6 335,089 - BPHYT_RS01465 0.38 +0.8 335,089 - BPHYT_RS01465 0.38 -0.2 335,089 - BPHYT_RS01465 0.38 -0.1 335,098 - BPHYT_RS01465 0.40 -1.4 335,098 - BPHYT_RS01465 0.40 +0.3 335,236 + BPHYT_RS01465 0.61 -0.1 335,236 + BPHYT_RS01465 0.61 -1.2 335,236 + BPHYT_RS01465 0.61 -0.8 335,236 + BPHYT_RS01465 0.61 -0.3 335,236 + BPHYT_RS01465 0.61 -0.8 335,236 + BPHYT_RS01465 0.61 +0.5 335,236 + BPHYT_RS01465 0.61 -0.4 335,236 + BPHYT_RS01465 0.61 -0.1 335,237 - BPHYT_RS01465 0.61 +0.2 335,237 - BPHYT_RS01465 0.61 -0.7 335,237 - BPHYT_RS01465 0.61 +0.1 335,513 + -0.9 335,514 - +2.0 335,520 + +1.0 335,520 + -0.7 335,520 + -0.7 335,525 + -0.3 335,526 - -1.7 336,604 + -0.1 336,605 - -0.7 336,694 - -0.8 336,694 - -0.6 336,739 - -1.7 336,739 - -1.0
Or see this region's nucleotide sequence