Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00805 and BPHYT_RS00810 overlap by 4 nucleotides BPHYT_RS00810 and BPHYT_RS00815 are separated by 14 nucleotides BPHYT_RS00815 and BPHYT_RS00820 overlap by 4 nucleotides
BPHYT_RS00805: BPHYT_RS00805 - type IV secretion system protein VirB10, at 181,925 to 183,127
_RS00805
BPHYT_RS00810: BPHYT_RS00810 - type IV secretion system protein VirB11, at 183,124 to 184,251
_RS00810
BPHYT_RS00815: BPHYT_RS00815 - hypothetical protein, at 184,266 to 184,676
_RS00815
BPHYT_RS00820: BPHYT_RS00820 - type IV secretory pathway protein VirD4, at 184,673 to 186,457
_RS00820
Position (kb) 183
184
185 Strain fitness (log2 ratio) -1
0
1
2 at 182.182 kb on - strand, within BPHYT_RS00805 at 182.242 kb on + strand at 182.292 kb on + strand, within BPHYT_RS00805 at 182.339 kb on + strand, within BPHYT_RS00805 at 182.340 kb on - strand, within BPHYT_RS00805 at 182.340 kb on - strand, within BPHYT_RS00805 at 182.343 kb on - strand, within BPHYT_RS00805 at 182.635 kb on + strand, within BPHYT_RS00805 at 182.844 kb on - strand, within BPHYT_RS00805 at 182.844 kb on - strand, within BPHYT_RS00805 at 182.958 kb on - strand, within BPHYT_RS00805 at 182.958 kb on - strand, within BPHYT_RS00805 at 183.098 kb on + strand at 183.099 kb on - strand at 183.099 kb on - strand at 183.171 kb on + strand at 183.172 kb on - strand at 183.638 kb on - strand, within BPHYT_RS00810 at 183.697 kb on - strand, within BPHYT_RS00810 at 183.714 kb on + strand, within BPHYT_RS00810 at 183.844 kb on + strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.959 kb on - strand, within BPHYT_RS00810 at 184.009 kb on + strand, within BPHYT_RS00810 at 184.010 kb on - strand, within BPHYT_RS00810 at 184.140 kb on + strand at 184.141 kb on - strand at 184.141 kb on - strand at 184.584 kb on + strand, within BPHYT_RS00815 at 184.745 kb on + strand at 184.813 kb on + strand at 184.957 kb on + strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.967 kb on + strand, within BPHYT_RS00820 at 185.225 kb on + strand, within BPHYT_RS00820 at 185.226 kb on - strand, within BPHYT_RS00820
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 182,182 - BPHYT_RS00805 0.21 -0.4 182,242 + +0.1 182,292 + BPHYT_RS00805 0.31 -0.4 182,339 + BPHYT_RS00805 0.34 -0.3 182,340 - BPHYT_RS00805 0.34 +0.2 182,340 - BPHYT_RS00805 0.34 -0.0 182,343 - BPHYT_RS00805 0.35 -1.3 182,635 + BPHYT_RS00805 0.59 -1.3 182,844 - BPHYT_RS00805 0.76 -0.6 182,844 - BPHYT_RS00805 0.76 +0.2 182,958 - BPHYT_RS00805 0.86 +0.5 182,958 - BPHYT_RS00805 0.86 -1.6 183,098 + -0.1 183,099 - -0.3 183,099 - +0.2 183,171 + +0.6 183,172 - -1.0 183,638 - BPHYT_RS00810 0.46 -0.7 183,697 - BPHYT_RS00810 0.51 +1.2 183,714 + BPHYT_RS00810 0.52 -1.0 183,844 + BPHYT_RS00810 0.64 -0.3 183,845 - BPHYT_RS00810 0.64 +0.0 183,845 - BPHYT_RS00810 0.64 +0.1 183,959 - BPHYT_RS00810 0.74 -0.3 184,009 + BPHYT_RS00810 0.78 +0.8 184,010 - BPHYT_RS00810 0.79 +2.2 184,140 + -0.1 184,141 - +0.2 184,141 - -1.2 184,584 + BPHYT_RS00815 0.77 +0.0 184,745 + +0.2 184,813 + -0.0 184,957 + BPHYT_RS00820 0.16 -0.2 184,958 - BPHYT_RS00820 0.16 +0.5 184,958 - BPHYT_RS00820 0.16 +0.4 184,967 + BPHYT_RS00820 0.16 +1.1 185,225 + BPHYT_RS00820 0.31 +0.3 185,226 - BPHYT_RS00820 0.31 -0.7
Or see this region's nucleotide sequence