Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00785 and BPHYT_RS00790 overlap by 4 nucleotides BPHYT_RS00790 and BPHYT_RS00795 are separated by 189 nucleotides BPHYT_RS00795 and BPHYT_RS00800 are separated by 14 nucleotides BPHYT_RS00800 and BPHYT_RS00805 overlap by 8 nucleotides
BPHYT_RS00785: BPHYT_RS00785 - conjugal transfer protein TraH, at 178,278 to 179,435
_RS00785
BPHYT_RS00790: BPHYT_RS00790 - conjugal transfer protein TraF, at 179,432 to 180,142
_RS00790
BPHYT_RS00795: BPHYT_RS00795 - conjugal transfer protein TraJ, at 180,332 to 181,090
_RS00795
BPHYT_RS00800: BPHYT_RS00800 - Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9), at 181,105 to 181,932
_RS00800
BPHYT_RS00805: BPHYT_RS00805 - type IV secretion system protein VirB10, at 181,925 to 183,127
_RS00805
Position (kb) 180
181
182 Strain fitness (log2 ratio) -1
0
1
2 at 179.381 kb on + strand at 179.532 kb on - strand, within BPHYT_RS00790 at 179.702 kb on - strand, within BPHYT_RS00790 at 179.702 kb on - strand, within BPHYT_RS00790 at 180.258 kb on - strand at 180.518 kb on + strand at 180.519 kb on - strand, within BPHYT_RS00795 at 180.642 kb on - strand, within BPHYT_RS00795 at 180.663 kb on - strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 181.005 kb on - strand, within BPHYT_RS00795 at 181.142 kb on + strand at 181.143 kb on - strand at 181.171 kb on + strand at 181.172 kb on - strand at 181.366 kb on + strand, within BPHYT_RS00800 at 181.366 kb on + strand, within BPHYT_RS00800 at 181.367 kb on - strand, within BPHYT_RS00800 at 181.430 kb on - strand, within BPHYT_RS00800 at 181.489 kb on + strand, within BPHYT_RS00800 at 181.490 kb on - strand, within BPHYT_RS00800 at 181.490 kb on - strand, within BPHYT_RS00800 at 181.597 kb on - strand, within BPHYT_RS00800 at 181.603 kb on + strand, within BPHYT_RS00800 at 181.676 kb on - strand, within BPHYT_RS00800
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 179,381 + -0.3 179,532 - BPHYT_RS00790 0.14 +1.9 179,702 - BPHYT_RS00790 0.38 +0.1 179,702 - BPHYT_RS00790 0.38 +0.7 180,258 - +0.2 180,518 + -0.6 180,519 - BPHYT_RS00795 0.25 +0.9 180,642 - BPHYT_RS00795 0.41 +0.3 180,663 - BPHYT_RS00795 0.44 -0.6 180,746 + BPHYT_RS00795 0.55 -0.2 180,746 + BPHYT_RS00795 0.55 +0.4 181,005 - BPHYT_RS00795 0.89 -0.3 181,142 + -0.9 181,143 - +0.2 181,171 + +0.2 181,172 - -0.6 181,366 + BPHYT_RS00800 0.32 -1.0 181,366 + BPHYT_RS00800 0.32 +0.5 181,367 - BPHYT_RS00800 0.32 +0.1 181,430 - BPHYT_RS00800 0.39 -0.3 181,489 + BPHYT_RS00800 0.46 -0.4 181,490 - BPHYT_RS00800 0.46 +0.7 181,490 - BPHYT_RS00800 0.46 -0.0 181,597 - BPHYT_RS00800 0.59 +1.3 181,603 + BPHYT_RS00800 0.60 -0.2 181,676 - BPHYT_RS00800 0.69 -0.1
Or see this region's nucleotide sequence