Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31430

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31420 and BPHYT_RS31425 are separated by 33 nucleotidesBPHYT_RS31425 and BPHYT_RS31430 are separated by 52 nucleotidesBPHYT_RS31430 and BPHYT_RS31435 are separated by 149 nucleotides BPHYT_RS31420: BPHYT_RS31420 - ABC transporter substrate-binding protein, at 2,603,846 to 2,604,658 _RS31420 BPHYT_RS31425: BPHYT_RS31425 - arginine ABC transporter ATP-binding protein, at 2,604,692 to 2,605,393 _RS31425 BPHYT_RS31430: BPHYT_RS31430 - FAD-dependent oxidoreductase, at 2,605,446 to 2,606,771 _RS31430 BPHYT_RS31435: BPHYT_RS31435 - LysR family transcriptional regulator, at 2,606,921 to 2,607,823 _RS31435 Position (kb) 2605 2606 2607Strain fitness (log2 ratio) -1 0 1 2at 2604.650 kb on + strandat 2604.996 kb on + strand, within BPHYT_RS31425at 2605.363 kb on - strandat 2605.428 kb on - strandat 2605.602 kb on - strand, within BPHYT_RS31430at 2606.240 kb on - strand, within BPHYT_RS31430at 2606.361 kb on - strand, within BPHYT_RS31430

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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2,604,650 + +2.1
2,604,996 + BPHYT_RS31425 0.43 +1.1
2,605,363 - -0.6
2,605,428 - +0.5
2,605,602 - BPHYT_RS31430 0.12 +0.0
2,606,240 - BPHYT_RS31430 0.60 +0.2
2,606,361 - BPHYT_RS31430 0.69 +0.4

Or see this region's nucleotide sequence