Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27055

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27050 and BPHYT_RS27055 are separated by 90 nucleotidesBPHYT_RS27055 and BPHYT_RS27060 are separated by 347 nucleotides BPHYT_RS27050: BPHYT_RS27050 - DNA topoisomerase, at 1,643,363 to 1,644,682 _RS27050 BPHYT_RS27055: BPHYT_RS27055 - hypothetical protein, at 1,644,773 to 1,645,066 _RS27055 BPHYT_RS27060: BPHYT_RS27060 - photosystem reaction center subunit H, at 1,645,414 to 1,645,911 _RS27060 Position (kb) 1644 1645 1646Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1644.080 kb on - strand, within BPHYT_RS27050at 1644.116 kb on + strand, within BPHYT_RS27050at 1644.308 kb on + strand, within BPHYT_RS27050at 1644.787 kb on + strandat 1645.082 kb on + strandat 1645.142 kb on + strandat 1645.142 kb on + strandat 1645.792 kb on + strand, within BPHYT_RS27060at 1645.795 kb on + strand, within BPHYT_RS27060at 1645.796 kb on - strand, within BPHYT_RS27060

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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1,644,080 - BPHYT_RS27050 0.54 +0.5
1,644,116 + BPHYT_RS27050 0.57 -0.5
1,644,308 + BPHYT_RS27050 0.72 +1.3
1,644,787 + -0.0
1,645,082 + +0.5
1,645,142 + +0.9
1,645,142 + -3.0
1,645,792 + BPHYT_RS27060 0.76 +1.0
1,645,795 + BPHYT_RS27060 0.77 +2.1
1,645,796 - BPHYT_RS27060 0.77 +1.2

Or see this region's nucleotide sequence