Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24335

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24330 and BPHYT_RS24335 are separated by 304 nucleotidesBPHYT_RS24335 and BPHYT_RS24340 are separated by 2 nucleotidesBPHYT_RS24340 and BPHYT_RS24345 are separated by 282 nucleotides BPHYT_RS24330: BPHYT_RS24330 - hisitidine kinase, at 1,024,599 to 1,025,453 _RS24330 BPHYT_RS24335: BPHYT_RS24335 - toxin HipA, at 1,025,758 to 1,027,098 _RS24335 BPHYT_RS24340: BPHYT_RS24340 - XRE family transcriptional regulator, at 1,027,101 to 1,027,355 _RS24340 BPHYT_RS24345: BPHYT_RS24345 - FAD-dependent oxidoreductase, at 1,027,638 to 1,028,978 _RS24345 Position (kb) 1025 1026 1027 1028Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1025.533 kb on + strandat 1025.533 kb on + strandat 1025.534 kb on - strandat 1025.534 kb on - strandat 1025.679 kb on - strandat 1025.695 kb on - strandat 1025.727 kb on + strandat 1025.727 kb on + strandat 1025.727 kb on + strandat 1025.728 kb on - strandat 1025.728 kb on - strandat 1026.087 kb on - strand, within BPHYT_RS24335at 1026.278 kb on + strand, within BPHYT_RS24335at 1026.302 kb on + strand, within BPHYT_RS24335at 1026.302 kb on + strand, within BPHYT_RS24335at 1026.303 kb on - strand, within BPHYT_RS24335at 1026.303 kb on - strand, within BPHYT_RS24335at 1026.388 kb on + strand, within BPHYT_RS24335at 1026.840 kb on - strand, within BPHYT_RS24335at 1026.999 kb on - strandat 1027.483 kb on - strandat 1027.502 kb on - strandat 1027.622 kb on - strandat 1027.622 kb on - strandat 1027.749 kb on + strandat 1027.752 kb on + strandat 1027.752 kb on + strandat 1027.753 kb on - strandat 1027.753 kb on - strandat 1027.753 kb on - strandat 1027.753 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
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1,025,533 + +0.0
1,025,533 + +0.1
1,025,534 - +0.3
1,025,534 - -3.2
1,025,679 - -0.4
1,025,695 - +0.6
1,025,727 + +0.1
1,025,727 + -0.4
1,025,727 + -0.0
1,025,728 - +0.4
1,025,728 - +1.2
1,026,087 - BPHYT_RS24335 0.25 +0.2
1,026,278 + BPHYT_RS24335 0.39 -0.5
1,026,302 + BPHYT_RS24335 0.41 -0.9
1,026,302 + BPHYT_RS24335 0.41 -0.7
1,026,303 - BPHYT_RS24335 0.41 -0.7
1,026,303 - BPHYT_RS24335 0.41 -2.0
1,026,388 + BPHYT_RS24335 0.47 -0.4
1,026,840 - BPHYT_RS24335 0.81 -0.1
1,026,999 - -0.1
1,027,483 - +1.4
1,027,502 - +0.5
1,027,622 - -0.9
1,027,622 - -0.2
1,027,749 + -0.2
1,027,752 + -0.2
1,027,752 + -0.5
1,027,753 - +2.1
1,027,753 - +0.3
1,027,753 - -1.0
1,027,753 - +0.3

Or see this region's nucleotide sequence