Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18780 and BPHYT_RS18785 overlap by 8 nucleotides BPHYT_RS18785 and BPHYT_RS18790 overlap by 7 nucleotides BPHYT_RS18790 and BPHYT_RS18795 are separated by 2 nucleotides BPHYT_RS18795 and BPHYT_RS18800 are separated by 70 nucleotides
BPHYT_RS18780: BPHYT_RS18780 - flagellar motor switch protein FliG, at 4,222,897 to 4,223,892
_RS18780
BPHYT_RS18785: BPHYT_RS18785 - flagellar assembly protein FliH, at 4,223,885 to 4,224,562
_RS18785
BPHYT_RS18790: BPHYT_RS18790 - ATP synthase, at 4,224,556 to 4,226,205
_RS18790
BPHYT_RS18795: BPHYT_RS18795 - flagellar export protein FliJ, at 4,226,208 to 4,226,657
_RS18795
BPHYT_RS18800: BPHYT_RS18800 - flagellar hook-length control protein, at 4,226,728 to 4,228,221
_RS18800
Position (kb) 4224
4225
4226
4227 Strain fitness (log2 ratio) -1
0
1 at 4223.581 kb on - strand, within BPHYT_RS18780 at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4224.398 kb on + strand, within BPHYT_RS18785 at 4225.586 kb on - strand, within BPHYT_RS18790 at 4225.807 kb on + strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.809 kb on + strand, within BPHYT_RS18790 at 4225.809 kb on + strand, within BPHYT_RS18790 at 4225.810 kb on - strand, within BPHYT_RS18790 at 4225.810 kb on - strand, within BPHYT_RS18790 at 4225.918 kb on + strand, within BPHYT_RS18790 at 4225.998 kb on + strand, within BPHYT_RS18790 at 4225.998 kb on + strand, within BPHYT_RS18790 at 4225.999 kb on - strand, within BPHYT_RS18790 at 4225.999 kb on - strand, within BPHYT_RS18790 at 4226.352 kb on + strand, within BPHYT_RS18795 at 4226.353 kb on - strand, within BPHYT_RS18795 at 4226.539 kb on - strand, within BPHYT_RS18795 at 4226.727 kb on + strand at 4226.728 kb on - strand at 4227.105 kb on + strand, within BPHYT_RS18800 at 4227.106 kb on - strand, within BPHYT_RS18800
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 4,223,581 - BPHYT_RS18780 0.69 +0.2 4,223,884 + -0.1 4,223,884 + +0.0 4,223,884 + +0.2 4,223,884 + -0.1 4,223,884 + -0.3 4,223,885 - +1.7 4,223,885 - +0.3 4,223,885 - +0.2 4,223,885 - -0.3 4,223,885 - -0.4 4,223,885 - -0.4 4,223,885 - +0.3 4,223,885 - -0.0 4,223,885 - +1.4 4,223,885 - +0.5 4,223,885 - -0.5 4,224,398 + BPHYT_RS18785 0.76 -0.1 4,225,586 - BPHYT_RS18790 0.62 +0.9 4,225,807 + BPHYT_RS18790 0.76 -0.8 4,225,808 - BPHYT_RS18790 0.76 +0.2 4,225,808 - BPHYT_RS18790 0.76 +0.3 4,225,808 - BPHYT_RS18790 0.76 +0.3 4,225,809 + BPHYT_RS18790 0.76 +0.8 4,225,809 + BPHYT_RS18790 0.76 -0.5 4,225,810 - BPHYT_RS18790 0.76 -0.1 4,225,810 - BPHYT_RS18790 0.76 +0.5 4,225,918 + BPHYT_RS18790 0.83 +0.1 4,225,998 + BPHYT_RS18790 0.87 +0.2 4,225,998 + BPHYT_RS18790 0.87 -0.4 4,225,999 - BPHYT_RS18790 0.87 -0.4 4,225,999 - BPHYT_RS18790 0.87 -1.0 4,226,352 + BPHYT_RS18795 0.32 -0.3 4,226,353 - BPHYT_RS18795 0.32 +1.1 4,226,539 - BPHYT_RS18795 0.74 +0.1 4,226,727 + +0.4 4,226,728 - +0.8 4,227,105 + BPHYT_RS18800 0.25 +0.2 4,227,106 - BPHYT_RS18800 0.25 +0.0
Or see this region's nucleotide sequence