Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13155

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBPHYT_RS13145 and BPHYT_RS13150 are separated by 20 nucleotidesBPHYT_RS13150 and BPHYT_RS13155 are separated by 291 nucleotidesBPHYT_RS13155 and BPHYT_RS13160 are separated by 100 nucleotidesBPHYT_RS13160 and BPHYT_RS13165 are separated by 107 nucleotidesBPHYT_RS13165 and BPHYT_RS13170 are separated by 243 nucleotides BPHYT_RS13145: BPHYT_RS13145 - chemotaxis protein CheY, at 3,002,491 to 3,002,919 _RS13145 BPHYT_RS13150: BPHYT_RS13150 - hisitidine kinase, at 3,002,940 to 3,003,572 _RS13150 BPHYT_RS13155: BPHYT_RS13155 - bacterioferritin, at 3,003,864 to 3,004,436 _RS13155 BPHYT_RS13160: BPHYT_RS13160 - membrane protein, at 3,004,537 to 3,004,698 _RS13160 BPHYT_RS13165: BPHYT_RS13165 - membrane protein, at 3,004,806 to 3,004,982 _RS13165 BPHYT_RS13170: BPHYT_RS13170 - ATPase, at 3,005,226 to 3,006,710 _RS13170 Position (kb) 3003 3004 3005Strain fitness (log2 ratio) -1 0 1 2 3at 3002.926 kb on - strandat 3002.971 kb on + strandat 3003.025 kb on + strand, within BPHYT_RS13150at 3003.556 kb on - strandat 3003.626 kb on + strandat 3003.627 kb on - strandat 3003.627 kb on - strandat 3003.627 kb on - strandat 3003.821 kb on + strandat 3003.822 kb on - strandat 3003.865 kb on + strandat 3003.866 kb on - strandat 3003.866 kb on - strandat 3003.938 kb on + strand, within BPHYT_RS13155at 3003.989 kb on - strand, within BPHYT_RS13155at 3004.004 kb on + strand, within BPHYT_RS13155at 3004.005 kb on - strand, within BPHYT_RS13155at 3004.278 kb on - strand, within BPHYT_RS13155at 3004.278 kb on - strand, within BPHYT_RS13155at 3004.538 kb on + strandat 3004.592 kb on + strand, within BPHYT_RS13160

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
remove
3,002,926 - -1.0
3,002,971 + +0.0
3,003,025 + BPHYT_RS13150 0.13 +0.3
3,003,556 - -0.3
3,003,626 + -0.7
3,003,627 - +1.2
3,003,627 - +2.8
3,003,627 - -0.1
3,003,821 + -1.1
3,003,822 - -0.6
3,003,865 + -0.5
3,003,866 - +0.1
3,003,866 - +1.2
3,003,938 + BPHYT_RS13155 0.13 +0.5
3,003,989 - BPHYT_RS13155 0.22 +1.3
3,004,004 + BPHYT_RS13155 0.24 -0.4
3,004,005 - BPHYT_RS13155 0.25 +0.2
3,004,278 - BPHYT_RS13155 0.72 +0.1
3,004,278 - BPHYT_RS13155 0.72 +0.1
3,004,538 + -1.4
3,004,592 + BPHYT_RS13160 0.34 -0.8

Or see this region's nucleotide sequence