Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02345 and BPHYT_RS02350 are separated by 97 nucleotides BPHYT_RS02350 and BPHYT_RS02355 are separated by 561 nucleotides
BPHYT_RS02345: BPHYT_RS02345 - enoyl-CoA hydratase, at 537,793 to 538,680
_RS02345
BPHYT_RS02350: BPHYT_RS02350 - hypothetical protein, at 538,778 to 539,599
_RS02350
BPHYT_RS02355: BPHYT_RS02355 - ferritin, at 540,161 to 541,033
_RS02355
Position (kb) 538
539
540 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 537.778 kb on + strand at 537.778 kb on + strand at 537.778 kb on + strand at 537.778 kb on + strand at 537.778 kb on + strand at 537.779 kb on - strand at 537.779 kb on - strand at 537.779 kb on - strand at 537.779 kb on - strand at 537.827 kb on - strand at 538.324 kb on + strand, within BPHYT_RS02345 at 538.324 kb on + strand, within BPHYT_RS02345 at 538.324 kb on + strand, within BPHYT_RS02345 at 538.324 kb on + strand, within BPHYT_RS02345 at 538.325 kb on - strand, within BPHYT_RS02345 at 538.325 kb on - strand, within BPHYT_RS02345 at 538.325 kb on - strand, within BPHYT_RS02345 at 538.327 kb on + strand, within BPHYT_RS02345 at 538.327 kb on + strand at 538.328 kb on - strand, within BPHYT_RS02345 at 538.698 kb on + strand at 538.699 kb on - strand at 538.758 kb on + strand at 539.597 kb on + strand at 539.597 kb on + strand at 539.785 kb on + strand at 539.785 kb on + strand at 539.786 kb on - strand at 539.786 kb on - strand at 539.788 kb on + strand at 539.788 kb on + strand at 539.791 kb on + strand at 539.791 kb on + strand at 539.791 kb on + strand at 539.791 kb on + strand at 539.792 kb on - strand at 539.792 kb on - strand at 539.792 kb on - strand at 539.794 kb on - strand at 539.795 kb on + strand at 539.795 kb on + strand at 539.795 kb on + strand at 539.796 kb on - strand at 539.802 kb on + strand at 539.963 kb on + strand at 540.075 kb on + strand at 540.075 kb on + strand at 540.075 kb on + strand at 540.105 kb on + strand at 540.115 kb on + strand at 540.115 kb on + strand at 540.115 kb on + strand at 540.160 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 remove 537,778 + -0.1 537,778 + -0.2 537,778 + -1.0 537,778 + +0.4 537,778 + -0.1 537,779 - +0.0 537,779 - -0.3 537,779 - -0.2 537,779 - +0.4 537,827 - +0.6 538,324 + BPHYT_RS02345 0.60 +1.1 538,324 + BPHYT_RS02345 0.60 +1.1 538,324 + BPHYT_RS02345 0.60 +0.3 538,324 + BPHYT_RS02345 0.60 +0.3 538,325 - BPHYT_RS02345 0.60 +0.5 538,325 - BPHYT_RS02345 0.60 -0.3 538,325 - BPHYT_RS02345 0.60 +0.0 538,327 + BPHYT_RS02345 0.60 -0.2 538,327 + -1.8 538,328 - BPHYT_RS02345 0.60 +1.8 538,698 + -0.0 538,699 - -1.4 538,758 + -0.2 539,597 + -0.3 539,597 + -0.5 539,785 + -0.1 539,785 + +0.5 539,786 - +1.3 539,786 - -1.0 539,788 + +0.1 539,788 + +0.1 539,791 + +0.1 539,791 + -0.1 539,791 + -0.1 539,791 + +0.4 539,792 - -0.6 539,792 - +0.4 539,792 - -1.3 539,794 - +0.5 539,795 + +1.0 539,795 + -0.0 539,795 + +0.2 539,796 - +3.0 539,802 + +0.1 539,963 + +0.1 540,075 + +0.2 540,075 + -0.2 540,075 + +1.1 540,105 + -0.4 540,115 + -0.5 540,115 + +0.2 540,115 + +0.4 540,160 + -1.3
Or see this region's nucleotide sequence