Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23865

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23855 and BPHYT_RS23860 are separated by 31 nucleotidesBPHYT_RS23860 and BPHYT_RS23865 are separated by 156 nucleotidesBPHYT_RS23865 and BPHYT_RS23870 are separated by 320 nucleotides BPHYT_RS23855: BPHYT_RS23855 - hypothetical protein, at 919,409 to 919,738 _RS23855 BPHYT_RS23860: BPHYT_RS23860 - malonic semialdehyde reductase, at 919,770 to 920,354 _RS23860 BPHYT_RS23865: BPHYT_RS23865 - 2OG-Fe(II) oxygenase, at 920,511 to 921,122 _RS23865 BPHYT_RS23870: BPHYT_RS23870 - LacI family transcription regulator, at 921,443 to 922,429 _RS23870 Position (kb) 920 921 922Strain fitness (log2 ratio) -2 -1 0 1at 919.583 kb on + strand, within BPHYT_RS23855at 919.584 kb on - strand, within BPHYT_RS23855at 919.584 kb on - strand, within BPHYT_RS23855at 919.719 kb on - strandat 920.241 kb on + strand, within BPHYT_RS23860at 920.241 kb on + strand, within BPHYT_RS23860at 920.454 kb on + strandat 920.455 kb on - strandat 920.718 kb on + strand, within BPHYT_RS23865at 920.719 kb on - strand, within BPHYT_RS23865at 920.719 kb on - strand, within BPHYT_RS23865at 920.725 kb on - strand, within BPHYT_RS23865at 920.816 kb on + strand, within BPHYT_RS23865at 921.190 kb on - strandat 921.358 kb on + strandat 921.556 kb on + strand, within BPHYT_RS23870at 921.557 kb on - strand, within BPHYT_RS23870at 921.771 kb on + strand, within BPHYT_RS23870at 922.100 kb on - strand, within BPHYT_RS23870at 922.101 kb on + strand, within BPHYT_RS23870

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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919,583 + BPHYT_RS23855 0.53 -2.0
919,584 - BPHYT_RS23855 0.53 -0.8
919,584 - BPHYT_RS23855 0.53 -0.4
919,719 - -1.6
920,241 + BPHYT_RS23860 0.81 +1.6
920,241 + BPHYT_RS23860 0.81 -0.1
920,454 + -1.0
920,455 - -0.1
920,718 + BPHYT_RS23865 0.34 +1.2
920,719 - BPHYT_RS23865 0.34 -0.3
920,719 - BPHYT_RS23865 0.34 -0.4
920,725 - BPHYT_RS23865 0.35 -0.9
920,816 + BPHYT_RS23865 0.50 -0.5
921,190 - -0.7
921,358 + +0.5
921,556 + BPHYT_RS23870 0.11 +0.2
921,557 - BPHYT_RS23870 0.12 +0.1
921,771 + BPHYT_RS23870 0.33 +0.5
922,100 - BPHYT_RS23870 0.67 -0.6
922,101 + BPHYT_RS23870 0.67 -0.4

Or see this region's nucleotide sequence