Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23380

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23370 and BPHYT_RS23375 are separated by 38 nucleotidesBPHYT_RS23375 and BPHYT_RS23380 are separated by 379 nucleotidesBPHYT_RS23380 and BPHYT_RS23385 are separated by 21 nucleotides BPHYT_RS23370: BPHYT_RS23370 - glycosyl transferase family 1, at 804,689 to 805,879 _RS23370 BPHYT_RS23375: BPHYT_RS23375 - hypothetical protein, at 805,918 to 806,409 _RS23375 BPHYT_RS23380: BPHYT_RS23380 - hypothetical protein, at 806,789 to 807,697 _RS23380 BPHYT_RS23385: BPHYT_RS23385 - group 1 glycosyl transferase, at 807,719 to 808,894 _RS23385 Position (kb) 806 807 808Strain fitness (log2 ratio) -1 0 1at 807.185 kb on + strand, within BPHYT_RS23380at 807.186 kb on - strand, within BPHYT_RS23380at 807.186 kb on - strand, within BPHYT_RS23380at 808.627 kb on - strand, within BPHYT_RS23385

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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807,185 + BPHYT_RS23380 0.44 -0.7
807,186 - BPHYT_RS23380 0.44 -0.1
807,186 - BPHYT_RS23380 0.44 -1.4
808,627 - BPHYT_RS23385 0.77 +0.6

Or see this region's nucleotide sequence