Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23265 and BPHYT_RS23270 are separated by 41 nucleotides BPHYT_RS23270 and BPHYT_RS23275 are separated by 81 nucleotides BPHYT_RS23275 and BPHYT_RS23280 are separated by 23 nucleotides BPHYT_RS23280 and BPHYT_RS23285 are separated by 25 nucleotides
BPHYT_RS23265: BPHYT_RS23265 - methylcrotonoyl-CoA carboxylase, at 783,565 to 785,172
_RS23265
BPHYT_RS23270: BPHYT_RS23270 - enoyl-CoA hydratase, at 785,214 to 785,999
_RS23270
BPHYT_RS23275: BPHYT_RS23275 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 786,081 to 788,159
_RS23275
BPHYT_RS23280: BPHYT_RS23280 - phosphate ABC transporter substrate-binding protein, at 788,183 to 788,974
_RS23280
BPHYT_RS23285: BPHYT_RS23285 - hypothetical protein, at 789,000 to 789,278
_RS23285
Position (kb) 786
787
788
789 Strain fitness (log2 ratio) -2
-1
0
1 at 785.658 kb on + strand, within BPHYT_RS23270 at 786.684 kb on + strand, within BPHYT_RS23275 at 786.747 kb on + strand, within BPHYT_RS23275 at 786.882 kb on + strand, within BPHYT_RS23275 at 787.095 kb on - strand, within BPHYT_RS23275 at 787.391 kb on + strand, within BPHYT_RS23275 at 788.200 kb on - strand at 788.408 kb on - strand, within BPHYT_RS23280 at 788.615 kb on - strand, within BPHYT_RS23280 at 788.635 kb on - strand, within BPHYT_RS23280 at 788.944 kb on + strand at 788.945 kb on - strand at 788.946 kb on + strand at 788.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 785,658 + BPHYT_RS23270 0.56 -1.0 786,684 + BPHYT_RS23275 0.29 -1.0 786,747 + BPHYT_RS23275 0.32 -0.9 786,882 + BPHYT_RS23275 0.39 -1.1 787,095 - BPHYT_RS23275 0.49 -0.6 787,391 + BPHYT_RS23275 0.63 -1.8 788,200 - -0.2 788,408 - BPHYT_RS23280 0.28 -0.1 788,615 - BPHYT_RS23280 0.55 +1.0 788,635 - BPHYT_RS23280 0.57 -0.7 788,944 + +0.5 788,945 - -0.1 788,946 + -0.5 788,947 - +0.1
Or see this region's nucleotide sequence