Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20635 and BPHYT_RS20640 are separated by 3 nucleotides BPHYT_RS20640 and BPHYT_RS20645 overlap by 4 nucleotides
BPHYT_RS20635: BPHYT_RS20635 - RND transporter, at 188,110 to 189,792
_RS20635
BPHYT_RS20640: BPHYT_RS20640 - multidrug efflux RND transporter permease subunit, at 189,796 to 192,963
_RS20640
BPHYT_RS20645: BPHYT_RS20645 - RND transporter, at 192,960 to 194,378
_RS20645
Position (kb) 189
190
191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2 at 188.863 kb on + strand, within BPHYT_RS20635 at 188.924 kb on - strand, within BPHYT_RS20635 at 189.064 kb on + strand, within BPHYT_RS20635 at 189.318 kb on + strand, within BPHYT_RS20635 at 189.318 kb on + strand, within BPHYT_RS20635 at 189.319 kb on - strand, within BPHYT_RS20635 at 189.946 kb on + strand at 190.030 kb on + strand at 190.031 kb on - strand at 190.031 kb on - strand at 190.031 kb on - strand at 190.063 kb on - strand at 190.079 kb on - strand at 190.283 kb on - strand, within BPHYT_RS20640 at 190.299 kb on - strand, within BPHYT_RS20640 at 191.207 kb on - strand, within BPHYT_RS20640 at 191.650 kb on + strand, within BPHYT_RS20640 at 191.651 kb on - strand, within BPHYT_RS20640 at 191.950 kb on - strand, within BPHYT_RS20640 at 192.058 kb on + strand, within BPHYT_RS20640 at 192.059 kb on - strand, within BPHYT_RS20640 at 192.154 kb on + strand, within BPHYT_RS20640 at 192.352 kb on + strand, within BPHYT_RS20640 at 192.354 kb on + strand, within BPHYT_RS20640 at 192.355 kb on - strand, within BPHYT_RS20640 at 192.358 kb on + strand, within BPHYT_RS20640 at 192.358 kb on + strand, within BPHYT_RS20640 at 193.419 kb on + strand, within BPHYT_RS20645 at 193.485 kb on + strand, within BPHYT_RS20645 at 193.876 kb on - strand, within BPHYT_RS20645
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 188,863 + BPHYT_RS20635 0.45 +0.4 188,924 - BPHYT_RS20635 0.48 +0.8 189,064 + BPHYT_RS20635 0.57 +0.7 189,318 + BPHYT_RS20635 0.72 -0.2 189,318 + BPHYT_RS20635 0.72 -1.0 189,319 - BPHYT_RS20635 0.72 -0.8 189,946 + -2.2 190,030 + -0.6 190,031 - +1.0 190,031 - +0.3 190,031 - +2.0 190,063 - -0.3 190,079 - -0.5 190,283 - BPHYT_RS20640 0.15 -0.5 190,299 - BPHYT_RS20640 0.16 -0.6 191,207 - BPHYT_RS20640 0.45 +0.3 191,650 + BPHYT_RS20640 0.59 +0.6 191,651 - BPHYT_RS20640 0.59 +1.0 191,950 - BPHYT_RS20640 0.68 -0.5 192,058 + BPHYT_RS20640 0.71 -0.2 192,059 - BPHYT_RS20640 0.71 -0.3 192,154 + BPHYT_RS20640 0.74 -0.7 192,352 + BPHYT_RS20640 0.81 +1.4 192,354 + BPHYT_RS20640 0.81 +0.1 192,355 - BPHYT_RS20640 0.81 +0.4 192,358 + BPHYT_RS20640 0.81 -0.0 192,358 + BPHYT_RS20640 0.81 -0.7 193,419 + BPHYT_RS20645 0.32 +0.4 193,485 + BPHYT_RS20645 0.37 -0.1 193,876 - BPHYT_RS20645 0.65 -0.4
Or see this region's nucleotide sequence