Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16710

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16705 and BPHYT_RS16710 are separated by 139 nucleotidesBPHYT_RS16710 and BPHYT_RS16715 are separated by 442 nucleotides BPHYT_RS16705: BPHYT_RS16705 - glutamate dehydrogenase, at 3,781,218 to 3,782,531 _RS16705 BPHYT_RS16710: BPHYT_RS16710 - LysR family transcriptional regulator, at 3,782,671 to 3,783,627 _RS16710 BPHYT_RS16715: BPHYT_RS16715 - adenylosuccinate lyase, at 3,784,070 to 3,785,458 _RS16715 Position (kb) 3782 3783 3784Strain fitness (log2 ratio) -3 -2 -1 0 1at 3782.964 kb on - strand, within BPHYT_RS16710at 3783.880 kb on - strandat 3783.921 kb on + strandat 3783.939 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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3,782,964 - BPHYT_RS16710 0.31 -3.7
3,783,880 - -2.4
3,783,921 + +1.0
3,783,939 + +0.6

Or see this region's nucleotide sequence