Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10750

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBPHYT_RS10740 and BPHYT_RS10745 are separated by 258 nucleotidesBPHYT_RS10745 and BPHYT_RS10750 are separated by 28 nucleotidesBPHYT_RS10750 and BPHYT_RS10755 are separated by 119 nucleotidesBPHYT_RS10755 and BPHYT_RS10760 overlap by 2 nucleotides BPHYT_RS10740: BPHYT_RS10740 - LuxR family transcriptional regulator, at 2,443,151 to 2,443,813 _RS10740 BPHYT_RS10745: BPHYT_RS10745 - FMN reductase, at 2,444,072 to 2,444,611 _RS10745 BPHYT_RS10750: BPHYT_RS10750 - LysR family transcriptional regulator, at 2,444,640 to 2,445,572 _RS10750 BPHYT_RS10755: BPHYT_RS10755 - hypothetical protein, at 2,445,692 to 2,445,892 _RS10755 BPHYT_RS10760: BPHYT_RS10760 - major facilitator transporter, at 2,445,891 to 2,447,213 _RS10760 Position (kb) 2444 2445 2446Strain fitness (log2 ratio) -3 -2 -1 0 1at 2443.911 kb on - strandat 2444.735 kb on + strand, within BPHYT_RS10750at 2445.655 kb on - strandat 2446.455 kb on + strand, within BPHYT_RS10760at 2446.537 kb on - strand, within BPHYT_RS10760

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
remove
2,443,911 - +0.2
2,444,735 + BPHYT_RS10750 0.10 -0.8
2,445,655 - -3.0
2,446,455 + BPHYT_RS10760 0.43 +0.3
2,446,537 - BPHYT_RS10760 0.49 -2.8

Or see this region's nucleotide sequence