Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS03680

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS03680 and BPHYT_RS03685 are separated by 185 nucleotidesBPHYT_RS03685 and BPHYT_RS03690 are separated by 19 nucleotides BPHYT_RS03680: BPHYT_RS03680 - tRNA-Lys, at 838,129 to 838,204 _RS03680 BPHYT_RS03685: BPHYT_RS03685 - Tol system periplasmic component YbgF, at 838,390 to 839,139 _RS03685 BPHYT_RS03690: BPHYT_RS03690 - membrane protein, at 839,159 to 839,668 _RS03690 Position (kb) 838 839Strain fitness (log2 ratio) -3 -2 -1 0 1at 837.453 kb on + strandat 837.453 kb on + strandat 837.454 kb on - strandat 837.565 kb on + strandat 837.617 kb on - strandat 837.739 kb on + strandat 837.739 kb on + strandat 837.740 kb on - strandat 837.743 kb on + strandat 837.916 kb on - strandat 838.285 kb on - strandat 838.371 kb on + strandat 838.383 kb on - strandat 838.392 kb on - strandat 838.446 kb on - strandat 838.557 kb on - strand, within BPHYT_RS03685at 838.598 kb on + strand, within BPHYT_RS03685at 838.652 kb on + strand, within BPHYT_RS03685at 839.157 kb on - strandat 839.160 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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837,453 + -0.1
837,453 + +0.1
837,454 - -1.7
837,565 + -0.0
837,617 - +1.4
837,739 + -0.1
837,739 + +0.7
837,740 - -0.6
837,743 + +0.6
837,916 - -0.7
838,285 - +0.0
838,371 + -0.0
838,383 - -1.0
838,392 - +0.6
838,446 - -0.4
838,557 - BPHYT_RS03685 0.22 +0.1
838,598 + BPHYT_RS03685 0.28 -0.9
838,652 + BPHYT_RS03685 0.35 -1.7
839,157 - -1.3
839,160 + -3.1

Or see this region's nucleotide sequence