Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03095 and BPHYT_RS03100 overlap by 31 nucleotides BPHYT_RS03100 and BPHYT_RS03105 are separated by 137 nucleotides BPHYT_RS03105 and BPHYT_RS03110 are separated by 31 nucleotides BPHYT_RS03110 and BPHYT_RS03115 are separated by 125 nucleotides
BPHYT_RS03095: BPHYT_RS03095 - alpha/beta hydrolase, at 692,694 to 693,650
_RS03095
BPHYT_RS03100: BPHYT_RS03100 - peptidase, at 693,620 to 694,462
_RS03100
BPHYT_RS03105: BPHYT_RS03105 - DSBA oxidoreductase, at 694,600 to 696,195
_RS03105
BPHYT_RS03110: BPHYT_RS03110 - MarR family transcriptional regulator, at 696,227 to 696,736
_RS03110
BPHYT_RS03115: BPHYT_RS03115 - amino acid deaminase, at 696,862 to 698,139
_RS03115
Position (kb) 694
695
696
697 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 693.651 kb on + strand at 693.652 kb on - strand at 693.679 kb on - strand at 693.749 kb on - strand, within BPHYT_RS03100 at 693.951 kb on + strand, within BPHYT_RS03100 at 694.537 kb on + strand at 694.537 kb on + strand at 694.556 kb on - strand at 694.644 kb on - strand at 694.738 kb on - strand at 694.771 kb on + strand, within BPHYT_RS03105 at 694.792 kb on + strand, within BPHYT_RS03105 at 694.793 kb on - strand at 694.793 kb on - strand, within BPHYT_RS03105 at 694.843 kb on + strand, within BPHYT_RS03105 at 694.846 kb on + strand, within BPHYT_RS03105 at 695.046 kb on - strand, within BPHYT_RS03105 at 695.113 kb on + strand, within BPHYT_RS03105 at 695.113 kb on + strand, within BPHYT_RS03105 at 695.113 kb on + strand, within BPHYT_RS03105 at 695.114 kb on - strand, within BPHYT_RS03105 at 695.491 kb on + strand, within BPHYT_RS03105 at 695.491 kb on + strand at 695.491 kb on + strand, within BPHYT_RS03105 at 695.637 kb on + strand, within BPHYT_RS03105 at 695.638 kb on + strand, within BPHYT_RS03105 at 695.638 kb on + strand, within BPHYT_RS03105 at 695.638 kb on + strand, within BPHYT_RS03105 at 695.638 kb on + strand, within BPHYT_RS03105 at 695.639 kb on - strand, within BPHYT_RS03105 at 695.892 kb on + strand, within BPHYT_RS03105 at 695.892 kb on + strand, within BPHYT_RS03105 at 695.892 kb on + strand, within BPHYT_RS03105 at 695.893 kb on - strand, within BPHYT_RS03105 at 695.893 kb on - strand, within BPHYT_RS03105 at 695.896 kb on + strand, within BPHYT_RS03105 at 695.896 kb on + strand, within BPHYT_RS03105 at 695.897 kb on - strand, within BPHYT_RS03105 at 695.997 kb on + strand, within BPHYT_RS03105 at 696.529 kb on + strand at 696.530 kb on - strand, within BPHYT_RS03110 at 696.530 kb on - strand, within BPHYT_RS03110 at 696.897 kb on - strand at 697.013 kb on + strand, within BPHYT_RS03115 at 697.014 kb on - strand, within BPHYT_RS03115 at 697.014 kb on - strand, within BPHYT_RS03115 at 697.095 kb on - strand, within BPHYT_RS03115 at 697.148 kb on - strand, within BPHYT_RS03115 at 697.148 kb on - strand, within BPHYT_RS03115 at 697.190 kb on + strand, within BPHYT_RS03115 at 697.190 kb on + strand, within BPHYT_RS03115 at 697.190 kb on + strand, within BPHYT_RS03115 at 697.191 kb on - strand, within BPHYT_RS03115 at 697.191 kb on - strand, within BPHYT_RS03115 at 697.191 kb on - strand, within BPHYT_RS03115
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 693,651 + -0.1 693,652 - -0.1 693,679 - +0.5 693,749 - BPHYT_RS03100 0.15 -0.7 693,951 + BPHYT_RS03100 0.39 -1.2 694,537 + +0.9 694,537 + +2.5 694,556 - +0.1 694,644 - -0.6 694,738 - -0.1 694,771 + BPHYT_RS03105 0.11 +2.0 694,792 + BPHYT_RS03105 0.12 -1.2 694,793 - +0.8 694,793 - BPHYT_RS03105 0.12 -0.9 694,843 + BPHYT_RS03105 0.15 -0.3 694,846 + BPHYT_RS03105 0.15 -0.2 695,046 - BPHYT_RS03105 0.28 -0.5 695,113 + BPHYT_RS03105 0.32 -0.1 695,113 + BPHYT_RS03105 0.32 -0.3 695,113 + BPHYT_RS03105 0.32 -0.7 695,114 - BPHYT_RS03105 0.32 -1.3 695,491 + BPHYT_RS03105 0.56 +0.3 695,491 + +0.9 695,491 + BPHYT_RS03105 0.56 -0.8 695,637 + BPHYT_RS03105 0.65 -0.7 695,638 + BPHYT_RS03105 0.65 -0.8 695,638 + BPHYT_RS03105 0.65 -2.9 695,638 + BPHYT_RS03105 0.65 -0.1 695,638 + BPHYT_RS03105 0.65 -0.4 695,639 - BPHYT_RS03105 0.65 -1.7 695,892 + BPHYT_RS03105 0.81 +0.3 695,892 + BPHYT_RS03105 0.81 -0.4 695,892 + BPHYT_RS03105 0.81 -0.5 695,893 - BPHYT_RS03105 0.81 -3.3 695,893 - BPHYT_RS03105 0.81 -1.5 695,896 + BPHYT_RS03105 0.81 +0.5 695,896 + BPHYT_RS03105 0.81 -1.0 695,897 - BPHYT_RS03105 0.81 -0.9 695,997 + BPHYT_RS03105 0.88 +0.1 696,529 + -0.2 696,530 - BPHYT_RS03110 0.59 -0.3 696,530 - BPHYT_RS03110 0.59 +2.9 696,897 - +0.6 697,013 + BPHYT_RS03115 0.12 +0.5 697,014 - BPHYT_RS03115 0.12 -0.3 697,014 - BPHYT_RS03115 0.12 -0.2 697,095 - BPHYT_RS03115 0.18 -0.9 697,148 - BPHYT_RS03115 0.22 +0.2 697,148 - BPHYT_RS03115 0.22 -0.7 697,190 + BPHYT_RS03115 0.26 -0.1 697,190 + BPHYT_RS03115 0.26 +0.3 697,190 + BPHYT_RS03115 0.26 -0.9 697,191 - BPHYT_RS03115 0.26 +0.5 697,191 - BPHYT_RS03115 0.26 +0.0 697,191 - BPHYT_RS03115 0.26 +1.1
Or see this region's nucleotide sequence