Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03055 and BPHYT_RS03060 are separated by 95 nucleotides BPHYT_RS03060 and BPHYT_RS03065 are separated by 384 nucleotides
BPHYT_RS03055: BPHYT_RS03055 - LysR family transcriptional regulator, at 681,210 to 682,097
_RS03055
BPHYT_RS03060: BPHYT_RS03060 - MFS transporter, at 682,193 to 683,512
_RS03060
BPHYT_RS03065: BPHYT_RS03065 - LuxR family transcriptional regulator, at 683,897 to 684,535
_RS03065
Position (kb) 682
683
684 Strain fitness (log2 ratio) -1
0
1 at 681.375 kb on + strand, within BPHYT_RS03055 at 681.375 kb on + strand, within BPHYT_RS03055 at 681.376 kb on - strand, within BPHYT_RS03055 at 681.443 kb on - strand, within BPHYT_RS03055 at 681.443 kb on - strand, within BPHYT_RS03055 at 681.521 kb on - strand, within BPHYT_RS03055 at 681.787 kb on + strand, within BPHYT_RS03055 at 681.790 kb on + strand, within BPHYT_RS03055 at 681.790 kb on + strand, within BPHYT_RS03055 at 681.790 kb on + strand, within BPHYT_RS03055 at 681.791 kb on - strand, within BPHYT_RS03055 at 681.791 kb on - strand, within BPHYT_RS03055 at 682.751 kb on + strand, within BPHYT_RS03060 at 682.751 kb on + strand at 682.752 kb on - strand, within BPHYT_RS03060 at 682.753 kb on + strand, within BPHYT_RS03060 at 682.753 kb on + strand, within BPHYT_RS03060 at 682.753 kb on + strand, within BPHYT_RS03060 at 683.025 kb on + strand, within BPHYT_RS03060 at 683.026 kb on - strand, within BPHYT_RS03060 at 683.111 kb on + strand, within BPHYT_RS03060 at 683.111 kb on + strand, within BPHYT_RS03060 at 683.113 kb on + strand, within BPHYT_RS03060 at 683.126 kb on - strand, within BPHYT_RS03060 at 683.126 kb on - strand, within BPHYT_RS03060 at 683.126 kb on - strand, within BPHYT_RS03060 at 683.406 kb on + strand at 683.536 kb on + strand at 683.669 kb on - strand at 684.270 kb on - strand, within BPHYT_RS03065 at 684.503 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 681,375 + BPHYT_RS03055 0.19 +1.3 681,375 + BPHYT_RS03055 0.19 +0.6 681,376 - BPHYT_RS03055 0.19 -0.5 681,443 - BPHYT_RS03055 0.26 +0.1 681,443 - BPHYT_RS03055 0.26 -0.1 681,521 - BPHYT_RS03055 0.35 -0.4 681,787 + BPHYT_RS03055 0.65 -0.5 681,790 + BPHYT_RS03055 0.65 +0.4 681,790 + BPHYT_RS03055 0.65 -0.7 681,790 + BPHYT_RS03055 0.65 -1.3 681,791 - BPHYT_RS03055 0.65 +0.0 681,791 - BPHYT_RS03055 0.65 -0.5 682,751 + BPHYT_RS03060 0.42 -1.2 682,751 + -1.0 682,752 - BPHYT_RS03060 0.42 -0.2 682,753 + BPHYT_RS03060 0.42 -0.4 682,753 + BPHYT_RS03060 0.42 -0.2 682,753 + BPHYT_RS03060 0.42 -0.5 683,025 + BPHYT_RS03060 0.63 -0.2 683,026 - BPHYT_RS03060 0.63 +0.1 683,111 + BPHYT_RS03060 0.70 -1.7 683,111 + BPHYT_RS03060 0.70 -0.3 683,113 + BPHYT_RS03060 0.70 +1.2 683,126 - BPHYT_RS03060 0.71 +0.0 683,126 - BPHYT_RS03060 0.71 -0.1 683,126 - BPHYT_RS03060 0.71 -1.3 683,406 + -0.3 683,536 + +0.2 683,669 - +0.9 684,270 - BPHYT_RS03065 0.58 -0.4 684,503 + -0.1
Or see this region's nucleotide sequence